基于混合SAMs包裹的纳米团簇和SPA的抗体固定方法用于糖化血红蛋白免疫场效应型传感器的制备(英文)  

Antibody Immobilization Based on Nano Spheres Modified by Mixed SAMs and SPA for HbA1c Immuno-FET Sensor Fabrication

作　　者：薛茜男[1,2] 边超[1] 佟建华[1] 孙楫舟[1] 张虹[1] 夏善红[1] 

机构地区：[1]中国科学院电子学研究所传感技术联合国家重点实验室,北京100190 [2]中国科学院研究生院,北京100080

出　　处：《纳米技术与精密工程》2011年第6期477-482,共6页Nanotechnology and Precision Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(2009CB320300)

摘　　要：本文提出了一种新型的抗体固定方法,并利用该方法制备了免疫场效应型传感器用于糖化血红蛋白水平的检测.首先,纳米金颗粒由混合自组装膜(SAMs)包裹形成一种功能化的纳米团簇.然后这种纳米团簇以自组装的方式固定在传感器金电极表面.之后,葡萄糖球菌蛋白A(SPA)被固定在纳米团簇表面用来定向固定抗体.利用该方法,纳米团簇可在金电极表面均匀分布.由此形成的多层生物膜可定向固定抗体,与抗体具有稳定的结合力,能较好地屏蔽噪声干扰,且与场效应型传感器兼容.利用该传感器对糖化血红蛋白和血红蛋白浓度检测,灵敏度分别为152.8μV.ng-1.mL和13.5μV.ng-1.mL.实验结果表明,该传感器具有较好的一致性和准确度,未来有望发展成为一种微型的用于糖尿病监控的糖化血红蛋白传感器.An antibody immobilization method was reported to fabricate an immuno-FET sensor for measuring hemoglobin Ale（HbAlc） level. Firstly, nanogold particles were capped with mixed self-assembled monolayers （SAMs） to form functional nano spheres. Then, the nano spheres were immobilized on the Au electrode of the immuno-FET sensor. After that, staphylococcal protein A （SPA） was immobilized on the nano spheres of electrode to further bond antibodies. In this way, nano spheres were distributed uniformly on Au electrode to form multi bionic film with the high ability to immobilize antibodies effectively and orientedly and to suppress noise interference. This biofilm was exactly suitable for the feature of FET sensor. Sensitivities of 152. 8 μV·ng^-1·mL for HbA1c and 13.5 μV·ng^-1·mL .mL for hemoglobin were exhibited by this sensor. Good consistency and accuracy were achieved. This sensor has the potential to develop into a micro HbA1 c sensor for the monitoring of diabetes.

关 键 词：混合自组装膜 纳米金 糖化血红蛋白 免疫传感器 葡萄糖球菌蛋白A 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]