手性流动相添加剂法拆分盐酸安非他酮对映体  

Chiral Separation of Bupropion Hydrochloride Enantiomers Using Mobile Phase Addtive

作　　者：孙玉杰[1] 苏立强[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化工时刊》2011年第10期15-17,20,共4页Chemical Industry Times

基　　金：黑龙江省自然科学基金项目(B200911)

摘　　要：以羧甲基-β-环糊精(carboxymethyl-β-cyclodextrin,简称CM-β-CD)为手性流动相添加剂,建立了盐酸安非他酮对映体拆分的高效液相色谱方法。系统考察了手性添加剂的种类及浓度、有机改性剂的比例、pH值和流速等因素对拆分的影响。结果表明:乙腈∶甲醇∶水40∶30∶30(体积比),pH4.16,CM-β-CD浓度为0.5 g.L-1,流速1.20 mL.min-1,对映体得到良好分离。该方法简单、快速、相对标准偏差小、重复性好。Separation of enantiomers of bupropion hydrochloride by HPLC was established using the carboxymethyl-β-cyclodextrin（CM-β-CD in short） as chiral mobile phase additive.The influences of the type and concentration of chiral mobile phase additive,the ratio of organic modifiers,the pH value and the flow rate on the enantiomers separation were investigated systematically.The results demonstrate that the better separation was achieved when the mobile phase consist of acetonitrile-methanol-water（40∶30∶30,ν/ν/ν,pH=4.16,containing 0.5 g·L-1 CM-β-CD） at the flow rate of 1.20 mL·min-1.The method was simple,fast,small relative standard deviations and considerably reproducible.

关 键 词：手性流动相添加剂 羧甲基-β-环糊精 盐酸安非他酮 

分 类 号：TQ463[化学工程—制药化工]