增强UV-B对黄连代谢及小檗碱含量的影响  

Effect of enhanced UV-B radiation on metabolism and berberine content of Coptis chinensis

作　　者：温泉[1] 张楠[1] 曹瑞霞[1] 周心渝[1] 唐娟[1] 吴能表[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《中国中药杂志》2011年第22期3063-3069,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30500041);中央高校基本科研业务费专项资金项目(XDJK2009C195)

摘　　要：目的:测定黄连根中小檗碱含量对不同UV-B辐射强度的响应,为提高黄连小檗碱含量提供理论参考。方法:实验设置了CK(自然光),UL(0.05 W.m-2),UM(0.10 W.m-2),UH(0.20 W.m-2)4个处理组,探讨UV-B辐射下初生代谢中光合特性、PPP途径和次生代谢中酪氨酸酶及黄连根中的小檗碱含量的变化特性。结果:在UH辐射胁迫下,黄连叶片中光合色素、PSII非光化学猝灭系数(qN),初始荧光(Fo),电子传递速率(ETR)以及6-磷酸葡萄糖脱氢酶活性、根中小檗碱含量均较其他组低。而处于UM辐射下的黄连,光合作用能力、PPP途径、酪氨酸酶活性以及小檗碱含量均高于对照组,产生了胁迫应激反应来抵御适度的UV-B辐射。结论:黄连处于UM辐射时,通过增强光合作用及PPP途径,能提供较多的次生代谢物前体物及次生代谢物所必需的NADPH,进而小檗碱的含量也相应提高,为黄连栽培种植中提高小檗碱含量提供参考。Objective： To reveal the response of content berberine in root of Coptis chinensis to different intensity of UV-B radiation,and provide the theory basis for promoting the content of berberine.Method： Four groups of UV-B radiation were set in the experiment which included： natural light control（0 W·m^-2）,UL（0.05 W·m^-2）,UM（0.10 W·m^-2）,UH（0.20 W·m^-2）.The special photosynthesis character,PPP pathway in the primary metabolism and lyrosinase activity,the changes of berberine in the root of C.chinensis were measured under different UV-B radiation.Result： Photosynthetic pigment,qN,Fo,ETR,activity of glucose-6-phosphate dehydrogenase and the content of berberine in the root of C.chinensis,all of these parameters were lower than other groups under the UH radiation.However,under the UM radiation,C.chinensis protected itself from the light UV-B radiation by promoting the power of photosynthesis and PPP pathway in order to produce more NADPH and secondary metabolites.Conclusion： C.chinensis increases its photosynthetic ability and PPP pathway which can furnish more precursor of secondary metabolites and NADPH that are needed in the secondary metabolism.Furthermore,the content of berberine increases correspondingly.The research provide the example for increasing the content of berberine in C.chinensis cultivation.

关 键 词：黄连 UV-B辐射 代谢 小檗碱 

分 类 号：R284[医药卫生—中药学]