人参皂苷Rg1延缓造血干细胞衰老过程中端粒长度和端粒酶活性的变化  

Changes of telomere and telomerase in effect of ginsenoside Rg1 to delay hematopoietic stem cell senescence

作　　者：周玥[1] 姜蓉[1] 杨斌[1] 姚欣[1] 王萍[1] 刘典锋[1] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室组织学与胚胎学教研室,重庆400016

出　　处：《中国中药杂志》2011年第22期3172-3175,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973818);重庆市科委自然科学基金重点项目(CSTC;2009BA5038)

摘　　要：目的:探讨端粒长度和端粒酶活性在人参皂苷Rg1延缓造血干细胞(HSC)衰老中的作用。方法:免疫磁性分选法分离纯化小鼠Sca-1+HSC后分5组:对照组,衰老模型组,Rg1组,Rg1治疗衰老组,Rg1延缓衰老组。衰老相关β-半乳糖苷酶(SA-β-Gal)染色、流式细胞术细胞周期测定和造血祖细胞混合集落(CFU-Mix)培养分析Rg1调控Sca-1+HSC衰老的生物学作用;Southern blotting与TRAP-PCR SYBR Green法检测端粒长度和端粒酶活性。结果:与衰老模型组相比,Rg1治疗组和Rg1延缓衰老组Sca-1+HSC的SA-β-Gal染色阳性细胞百分比降低,G1期细胞比例下降,生成CFU-Mix数相对增加,端粒长度缩短明显减少,端粒酶活性增强;Rg1延缓衰老组各检测指标变化均较Rg1治疗组明显。结论:Rg1可能通过激活端粒酶活性和减少端粒长度缩短发挥延缓和治疗Sca-1+HSC衰老的作用。Objective： To investigate the roles of telomere and telomerase in the effect of ginsenoside Rg1 to delay hematopoietic stem cell senescence.Method： Sca-1^＋HSC was isolated by magnetic cell sorting（MACS） and divided into five groups： the control group,the aged model group,the Rg1 group,the Rg1 treated aged group and the Rg1 delayed aged group.The changes of cells were observed by senescence-associated β-Galactosidase（SA-β-Gal） staining.Cell cycle assay and culture of mixed hematopoietic progenitor cell were used to investigate the effect of ginsenoside Rg1 to delay Sca-1^＋HSC senescence.Telomere length and telomerase activity were detected by southern blotting and TRAP-PCR-SYBR Green staining.Result： Compared with aged model group,the percentage of positive cells expressed SA-β-Gal and the number of cells entered G1 phase were decreased and the number of colony of mixed hematopoietic progenitor was increased.It showed markedly decreased in the shortening of telomere length and reinforcing in the telomerase activity to Rg1 treated aged group and Rg1 delayed aged group.The change of Rg1 delayed aged group was significantly higher than Rg1 treated aged group.Conclusion： Activation of telomerase and prolonging of telomere length might be involved in the process of ginsenoside Rgl to delay and treat the senescence of Sca-1^＋HSC.

关 键 词：人参皂苷RG1 造血干细胞 衰老 端粒 端粒酶 

分 类 号：R285.5[医药卫生—中药学]