GL50分子在活化T细胞表面的表达及对细胞增殖的影响  

Expression of GL50 molecule on activated T Cells and its effect on the cell proliferation

作　　者：孙中文[1,2] 黄静芳[1] 陶鸿[1] 易丽娴[1] 孙静[1] 肖洋[1] 

机构地区：[1]苏州卫生职业技术学院,江苏苏州215009 [2]苏州市检验医学生物技术重点实验室

出　　处：《山东医药》2011年第43期12-13,共2页Shandong Medical Journal

基　　金：江苏省卫生厅医学科技基金项目(Z201015);苏州卫生职业技术学院医学科研基金资助项目(szwzy200605)

摘　　要：目的探讨GL50分子在活化T细胞表面的表达及其生物学意义。方法分别取不同时相(24～144 h)激发型CD3单抗联合CD28单抗活化的T细胞,采用流式细胞术分析T细胞表面GL50分子表达阳性率,RT-PCR法检测T细胞内GL50分子mRNA转录水平;3H-TdR掺入法分析阻断GL50信号对T细胞增殖的影响。结果激发型CD3单抗联合CD28单抗活化T细胞后24 h GL50分子阳性表达率为17.2%,其后逐渐升高至活化120 h时达最大阳性率95.7%,并维持至活化144 h时基本不变;T细胞活化24～144 h均可检测到GL50分子mRNA表达;阻断性GL50分子单抗对活化T细胞增殖具有显著抑制作用。结论 GL50分子在活化T细胞表面呈诱导性表达,且可与T细胞表面自身受体结合在免疫应答放大效应中发挥重要作用。Objective To explore the expression of GL50 on activated T cells and its biological significance. Methods Time courses of T cells activated by agonistic anti-CD3 plus anti-CD28 were chosen, then the positive rate of GL50 expression was analyzed through flow cytometry,the level of GL50 mRNA analyzed by RT-PCR. Effects of GL50 on T cells were measured by ^3H-TdR incorporation. Results The positive percentage of GL50 on activated T cells at 24 h was 17.2% , and then rapidly increased to 95.7% at 120 h after activation,and which maintained stable to the 144 h; the expression of GL50 mRNA could be detected at 24-144 h after the activation of T cells; antagonist GL50 mAbs could inhibit the proliferation of T cells. Conclusion GL50 molecule can express inductively on activated T cells, and play an important role during the course of immune response through interaction of its corresponding receptor.

关 键 词：活化T细胞 GL50分子 生物学意义 

分 类 号：R446.62[医药卫生—诊断学]