PAR-1作为口腔舌鳞癌治疗潜在靶点的临床研究  

The clinical research of PAR-1 as a potentially therapeutic target in oral tongue squamous cell carcinoma

作　　者：卢军[1] 朱惠君[2] 程红霞[1] 施公胜[2] 黄剑飞[2] 

机构地区：[1]南通大学附属医院口腔科科,江苏南通226001 [2]南通大学附属医院病理科,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2011年第10期1488-1492,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:观察并分析蛋白酶激活受体-1(proteinase activated receptor-1,PAR-1)基因在口腔舌鳞癌(oral tongue squamouscell carcinoma,OTSCC)中的表达情况及其临床意义,探讨PAR-1作为OTSCC潜在治疗靶点的可能性。方法:用实时定量RT-PCR检测15例OTSCC组织及癌旁舌组织中PAR-1 mRNA的表达,用免疫组化检测PAR-1蛋白在109例OTSCC组织及其癌旁舌组织中的表达情况。结果:PAR-1 mRNA及蛋白在OTSCC组织中的表达明显高于其在癌旁舌组织中的表达(P<0.001);PAR-1蛋白高表达出现于OTSCC肿瘤直径较大和TNM分期晚的患者,在统计学上有差异(P<0.05);随着OTSCC分化程度的降低、淋巴结转移,PAR-1蛋白表达也随之增高,但无明显统计学差异(P>0.05);PAR-1蛋白在OTSCC组织中的表达与患者性别、年龄无关。经Kaplan-Meier法生存曲线和COX比例风险模型分析,结果显示PAR-1高表达和TNM分期晚是OTSCC患者预后不良的独立因素。结论:PAR-1高表达与OTSCC的不良预后有关,可作为临床预后危险因素之一,也可作为靶向治疗OTSCC的潜在靶点。Objective： To investigate the expression and clinical significance of proteinase activated receptor-1（PAR-1） in oral tongue squamous cell carcinoma（OTSCC）.Methods： Real-time RT-PCR was used to characterize the expression of PAR-1 mRNA in 15 samples of OTSCC.The expression of PAR-1 protein in 109 tumor tissue samples including with peritumoral tissue from patients with OTSCC was detected with immunohistochemisty.Results： RT-PCR and immunohistochemisty showed that the expression of PAR-1 in OTSCC was significantly higher than that in the tumor-adjacent non-malignant tissues（P 0.01）.And the statistical analysis of clinicopathologic characteristics and immunohistochemistry showed that the high expression of PAR-1 protein in OTSCC was related to diameter of tumor and the later of stage grouping with TNM classification（P = 0.035 and P = 0.015,respectively）.In contrast,no statistically significant correlation was found for gender and age.Kaplan-Meier survival and Cox regression analyses also showed that high PAR-1 expression and stage grouping with TNM were independent poor prognostic factors for OTSCC patients.Conclusion： The expression of PAR-1 protein was related with malignant level of OTSCC cancer.PAR-1 could be used as a mark of clinical prognosis and a potentially therapeutic target of OTSCC.

关 键 词：PAR-1 舌鳞癌 实时定量PCR 免疫组化 靶向治疗 

分 类 号：Q786[生物学—分子生物学]