高效液相色谱编程荧光法测定室尘中痕量多环芳烃  

Determination of polycyclic aromatic hydrocarbons in indoor dust by high-performance liquid chromatography with fluorescence detection and wavelength programming

作　　者：陆晓梅[1] 王炳玲[2] 张晓玲[1] 张绮[2] 

机构地区：[1]南京医科大学公共卫生学院卫生检验学系,江苏南京210029 [2]南京医科大学职业医学与环境卫生学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第10期1521-1526,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(81072268);江苏省自然科学基金面上项目(BK2010535)

摘　　要：目的:建立高效液相色谱法测定室尘中16种常见多环芳烃(PAHs)的最佳分离条件及测定的最佳荧光激发、发射波长和增益。方法:用吸尘器采集室尘,用丙酮超声提取室尘中的多环芳烃化合物,浓缩后用乙腈定容,乙腈和水为流动相,进行高效液相色谱梯度淋洗分离,程序波长荧光检测器检测。结果:除苊烯外的15种多环芳烃化合物在28 min内得到了很好的分离,在0.5～50 ng/ml范围内线性关系良好,测定的相对标准偏差为1.35%～5.89%,加标回收率为62.45%～110.36%。结论:该方法测定室尘中多环芳烃化合物快速简便、灵敏、重现性好。Objective： To find out the optimal separating conditions and wavelength program for measurement of 16 polycyclic aromatic hydrocarbons in house dust by high-performance liquid chromatography（HPLC） with fluorescence detector.Methods： Vacuum cleaner was used for gathering the house dust,and acetone was added and extraction was performed in a ultrasonic bath.The supernatant was concentrated and then added methyl cyanide to 0.1 ml.With methyl cyanide and water as mobile phase,PAHs were efficiently separated by gradient elution and detected by fluorescence detector and wavelength programming.Results： Except for acenaphthylene,15 PAHs had highly efficient separation within 28 min and showed good linearity with 0.5-50 ng/ml range.The relative standard deviations were 1.35%-5.89%,and the recoveries were 62.45%-110.36%.Conclusion： HPLC with fluorescence detection and wavelength programming could be used for detection of PAHs in house dust because of its quickness,simplicity,sensitivity and reproducibility.

关 键 词：高效液相色谱法 室尘 多环芳烃 

分 类 号：R917[医药卫生—药物分析学]