新生大鼠七氟醚麻醉对发育期认知功能和海马乙酰胆碱酯酶活性的影响  

Effect of sevoflurane anesthesia in the neonatal period on cognitive function and acetylcholinesterase activity in hippocampus in developmental stages in rats

作　　者：徐桂萍[1] 唐冬梅[1] 张永杰[1] 

机构地区：[1]新疆维吾尔自治区人民医院麻醉科,乌鲁木齐市830001

出　　处：《中华麻醉学杂志》2011年第8期961-963,共3页Chinese Journal of Anesthesiology

基　　金：新疆维吾尔自治区自然科学基金（2011211A056）

摘　　要：目的探讨新生大鼠七氟醚麻醉对发育期认知功能和海马乙酰胆碱酯酶（AChE）活性的影响。方法出生7d的SD大鼠80只，体重12～16g，雌雄各半，采用随机数字表法，将其随机分为5组（n=16）：A组和B组分别吸入3％七氟醚6h和2h；C组和D组分别吸入1．5％七氟醚6h和2h；E组只吸入氧气。待大鼠生长至断乳期（出生后16—21d）和性成熟期（出生后55～60d）时，采用Morris水迷宫实验测试认知功能。分别于出生后21和60d水迷宫实验结束后30min处死大鼠，取海马组织，采用比色法测定AChE活性。结果与E组比较，A组出生后17～20d、B组和C组出生后18、19d、D组出生后18d时逃避潜伏期延长，A组出生后21d时AChE活性升高（P〈0．05或0．01）；与A组比较，B组和C组出生后19、20d时逃避潜伏期缩短，出生后21d时海马AChE活性降低（P〈0．05或0．01）；与B组和C组比较，D组出生后19、20d时逃避潜伏期缩短（P〈0．05）；五组大鼠原平台象限活动时间和穿越原平台次数、出生后60d时AChE活性比较差异无统计学意义（P〉0．05）；性成熟期各组逃避潜伏期差异无统计学意义（P〉0．05）。结论新生大鼠接受七氟醚麻醉后，可短暂降低发育期认知功能，且与吸入浓度和时间有关，其机制可能与增强海马AChE活性有关。Objective To investigate the effect of sevoflurane anesthesia in the neonatal period on cognitive function and acetylcholinesterase（AChE） activity in hippocampus in developmental stages in rats. Methods Eighty 7 day-old SD rats weighing 12-16 g were randomly divided into 5 groups（ n = 16 each） ：groups A and B inhaled 3 % sevoflurane in oxygen for 6 and 2 h respectively ; groups C and D inhaled 1.5 % sevoflurane in oxygen for 6 and 2 h respectively ; group E inhaled oxygen only. The cognitive function was assessed using Morris water maze test in weaning period（16-21 day-old） and sexual maturity period （55-60 day-old） respectively. The rats were sacrificed at 30 min after Morris water maze test was finished at 21 and 60 d after birth, and the hippoeampus were removed for determination of AChE activity using colorimetry method. Results Compared with group E, escape latency was prolonged during 17-20 d after birth in group A, and at 18,19 d after birth in groups B and C, and at 18 d after birth in group C,and AChE activity increased at 21 d after birth in group A（ P 〈 0.05 or 0.01）. Compared with group A, escape latency was shortened at 19,20 d after birth, and AChE activity decreased at 21 d after birth in groups B and C（P 〈 0.05 or 0.01）. Compared with groups B and C, escape latency was shortened at 19,20 d after birth in group D（ P 〈 0.05）. There were no significantly differences in probe time in original platform quadrant, frequency of crossing the original platform and AChE activity at 60 d after birth and escape latency during sexual maturity period among the 5 groups （ P 〉 0.05）. Conclusion Sevoflurane anesthesia in the neonatal period can reversible decrease the congnitive function in developmental stages in rats in concentration and time dependent manners, and the mechanism may be related to increasing the AChE activity in hippocampus.

关 键 词：麻醉药 吸入 麻醉 婴儿 新生 儿童发育 认知障碍 乙酰胆碱酯酶 海马 

分 类 号：R965[医药卫生—药理学]