心肌钙敏感受体在高位脊髓损伤大鼠心肌损伤中的作用  

Role of myocardial calcium-sensing receptor in a rats model of high-level spinal cord injury

作　　者：陈辉[1] 马骋[1] 张小霓[1] 巩超[1] 林财珠[1] 

机构地区：[1]福建医科大学附属第一医院麻醉科,福州市350005

出　　处：《中华麻醉学杂志》2011年第8期992-994,共3页Chinese Journal of Anesthesiology

基　　金：福建省卫生厅创新课题（2009-XB-24）

摘　　要：目的探讨心肌钙敏感受体（CaSR）在高位脊髓损伤大鼠心肌损伤中的作用。方法健康雄性SD大鼠18只，体重250～300g，采用随机数字表法，将大鼠随机分为2组：假手术组（S组，n=6）和高位脊髓损伤组（SCI组，n=12）。采用砝码（10g）从5cm高处沿中空玻璃管垂直自由落下撞击C7脊髓的方法制备高位脊髓损伤模型。SCI组于高位脊髓损伤后12和24h（T1,2）时，S组于T1时取血样，测定血清肌酸激酶（CK）和CK-MB活性，取心肌组织，在透射电镜下观察心肌超微结构，分别采用荧光定量PCR法和Western blot法检测心肌CaSRmRNA及其蛋白的表达水平。结果与s组比较，SCI组血清CK和CK-MB活性升高，心肌CaSRmRNA及其蛋白表达上调（P〈0．05）；与T1时比较，SCI组T2时血清CK活性升高，CK-MB活性降低，心肌CaSRmRNA表达上调（P〈0．05），CaSR蛋白表达差异则无统计学意义（P〉0．05）。SCI组T1,2时心肌超微结构呈现不同程度的损伤改变。结论大鼠高位脊髓损伤后心肌CaSR表达上调，该变化可能是高位脊髓损伤后心肌损伤的机制之一。Objective To investigate the role of myocardial calcium-sensing receptor （CaSR） in a rat model of high-level spinal cord injury （SCI） .Methods Eighteen healthy male SD rats weighing 250-300 g were randomly divided into 2 groups： sham operation group（group S, n = 6） and SCI group（ n = 12）. SCI model was induced by dropping a 10 g weight onto spinal cord （C7） in freely vertical falling along the hollow glass tube from 5 cm height. The blood samples were taken 12 and 24 h after SCI in group SCI and 12 h after SCI in group S, and serum activity of creatine kinase（CK） and MB isoenzyme of creatine kinase（CK-MB） were measured. Then myo- cardium specimens were obtained for ultrastructure examination and determination of CaSR mRNA and protien ex- pression by fluorescence quantitative RCR and Western blot. Results Serum activities of CK and CK-MB and CaSR mRNA and protein expression were higher in group SCI than in group S. Serum activity of CK and CaSR mRNA expression were higher,and serum activity of CK-MB was lower at 24 h after SCI than that at 12 h after SCI. There was no significantly difference in CaSR protein expression between the two time points in group SCI. The ultrastructure examination showed that myocardial injury was found in group SCI. Conclusion The expression of CaSR is up-regulated after SCI in rats, which might be the mechanism of myocardial injury after SCI.

关 键 词：受体 钙敏感 脊髓损伤 心肌 

分 类 号：R651.2[医药卫生—外科学]