再灌注期间给予富氢液对大鼠全脑缺血再灌注损伤的影响  

Effects of hydrogen-rich saline given during reperfusion on global cerebral ischemia-reperfusion injury in rats

作　　者：惠康丽[1] 韩云飞[2] 嵇晴[1] 孙学军[3] 段满林[1] 

机构地区：[1]南京军区南京总医院麻醉科,210002 [2]南京军区南京总医院训练中心,210002 [3]第二军医大学潜水医学系

出　　处：《中华麻醉学杂志》2011年第8期1009-1012,共4页Chinese Journal of Anesthesiology

基　　金：军区医药卫生科研基金（06MA131）

摘　　要：目的评价再灌注期间给予富氢液对大鼠全脑缺血再灌注损伤的影响。方法成年雄性SD大鼠72只，月龄2．0-2．5个月，体重260～300g，采用随机数字表法，将其随机分为3组（n=24）：假手术组（S组）、脑缺血再灌注组（I／R组）和富氢液组（H组）。I／R组和H组采用四血管阻塞法（缺血15min）制备全脑缺血再灌注损伤模型。H组于再灌注6h时腹腔注射0．6mmol／L富氢液5ml／kg，I／R组给予等容量生理盐水。再灌注24h时，每组处死18只大鼠，取海马组织，测定丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介索．6（IL-6）含量、NF-κB活性、活化的caspase-3表达；再灌注72h时，处死6只大鼠，取脑组织，HE染色，光镜下观察海马CA1区病理学结果，进行海马CA1区正常锥体细胞计数。结果与S组比较，I／R组海马组织MDA、TNF-α、IL-6含量和NF-κB活性升高，活化caspase-3表达上调，正常锥体细胞计数减少（P〈0．05）；与I／R组比较，H组海马组织MDA、TNF—α、IL-6含量和NF-κB活性降低，活化caspase-3表达下调，正常锥体细胞计数增多（P〈0．05）。H组脑组织海马CA1区病理学损伤程度轻于I／R组。结论再灌注期间给予富氢液可减轻大鼠全脑缺血再灌注损伤，其机制与抑制脂质过氧化反应、炎性反应和细胞凋亡有关。Objective To evaluate the effect of hydrogen-rich saline given during reperfusion on global cerebral ischemia-reperfusion （I/R） injury in rats. Methods Seventy-two adult male SD rats, aged 2.0-2.5 months, weighing 260-300 g, were randomly divided into 3 groups （ n = 24 each） ： sham operation group （group S）, group I/R and hydrogen-rich saline group （group H）. In groups I/R and H cerebral I/R was induced by occlusion of 4 vessels（ cauterization of bilateral carotid arteries and 15 min occlusion of bilateral common carotid arteries）. In group H intraperitoneal 0.6 mmol/L hydrogen-rich saline 5 ml/kg was injected at 6 h of reperfusion, while equal volume of normal saline was injected instead of hydrogen-rich saline. Eighteen rats of each group were sacrificed at 24 h of reperfusion, and then the hippocampi were removed for determination of malondialdehyde （MDA）, tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）contents, and nuclear factor-κB （NF-κB） activity and activated caspase-3 expression. Another six rats of each group were sacrificed at 72 h of reperfusion, and then brain tissues were removed for microscopic examination and counting the number of uninjured pyramidal cells in hippocampal CA1 region. Results Compared with S group, the contents of MDA, TNF-α, IL-6 and NF-κB activity were significantly increased, activated caspase-3 expression was significantly up-regulated, uninjured pyramidal cells in hippocampal CA1 region were significantly decreased in I/R group（ P 〈 0.05）. Hydrogen-rich saline given during reperfusion attenuated the above-mentioned I/R-induced changes（ P 〈 0.05 ）. The histologic damage of the hippocampal CA1 region was significantly slighter in group H than group I/R. Conclusion Hydrogen-rich saline given during reperfusion can reduce global cerebral I/R injury in rats through inhibition of lipid peroxidation, inflammatory response and apoptosis.

关 键 词：氢 再灌注损伤 脑 

分 类 号：R285.5[医药卫生—中药学]