血管内皮生长因子在糖尿病股骨头多种组织的旁分泌作用  

Paracrine action of VEGF upon femoral-head different tissues of diabetic rat

作　　者：陈海英[1] 陈建明[1] 陈少强[2] 刘文革[3] 唐军民[4] 

机构地区：[1]福建莆田学院医学院基础医学部,福建莆田351100 [2]福建医科大学人体解剖学与组织胚胎学系,福建福州350003 [3]福建医科大学附属协和医院骨科,福建福州350003 [4]北京大学医学部人体解剖与组织胚胎学系,北京100191

出　　处：《解剖科学进展》2011年第6期586-589,共4页Progress of Anatomical Sciences

基　　金：福建教育厅科研基金资助项目(No.Jb08222)

摘　　要：目的探讨血管内皮生长因子(Vascular endothelial growth factor,VEGFmRNA)对糖尿病股骨头不同组织的旁分泌作用。方法建立速发型链脲佐菌素(streptozotocin,STZ)糖尿病大鼠模型,随机分为正常5周组(CON1)、10周组(CON2)及15周组(CON3),糖尿病5周组(DM1)、10周组(DM2)及15周组(DM3),每组10只。对各组大鼠股骨头关节软骨、生长板、股骨头松质骨光镜下作组织病理学分析;观察分析血管内皮生长因子(VEGFmRNA)在不同组织原位杂交的表达强度。结果 VEGFmRNA在对照各组的关节软骨均有表达,糖尿病组随病程延长,关节软骨与松质骨VEGFmRNA表达均高于正常组(<0.01)。糖尿病15周组生长板VEGFmRNA表达均高于正常组(<0.01)。糖尿病各组股骨头松质骨VEGFmRNA表达逐渐增强(<0.05)。结论糖尿病大鼠股骨头关节软骨VEGFmRNA旁分泌功能最活跃,可能参与糖尿病发病。Objective To explore the paracrine action of VEGF upon femoral-head different tissues of diabetic rat.Methods The diabetic model（STZ）rats were established by administration of streptozotocin（STZ）.Sixty animals were divided averagely into the normal groups（CON1,CON2 and CON3）and the diabetic groups（DM1,DM2 and DM3）.The expression of VEGFmRNA was studied with in situ hybridization and analyzed by microimage analysis system.Results VEGFmRNA in the articular cartilage was expressed in all control group,the expression level of VEGFmRNA was higher in diabetic group than in control group（P〈0.05）as illness prolonged.The expression level of VEGFmRNA in the growth plate was increased（P〈0.01）in 15-week diabetic group than in control,increased in the trabecular bone in the 10 and 15-week diabetic group than in control（P〈0.05）.Conclusion The active paracrine action of VEGFmRNA at a articular cartilage might be involved in the pathogenesis of diabetis.

关 键 词：糖尿病 血管内皮生长因子 股骨头 原位杂交 旁分泌 大鼠 

分 类 号：R587.2[医药卫生—内分泌]