EDTA纸片法和三维试验检测阴沟肠杆菌AmpC酶的比较  

Comparison of EDTA disk test with three-dimensional test in detection of AmpC-producing Enterobacter cloacae isolates

作　　者：胡玉华 卢月梅[1,2] 吴劲松 吴伟元[1,2] 李文青 张阮章[1,2] 

机构地区：[1]深圳市人民医院 [2]暨南大学第二临床学院,广东深圳518020

出　　处：《中国微生态学杂志》2011年第11期1027-1028,共2页Chinese Journal of Microecology

摘　　要：目的比较EDTA纸片法及头孢西丁三维试验检测阴沟肠杆菌AmpC酶的符合程度。方法收集近几年阴沟肠杆菌株97株临床菌株,应用EDTA纸片法和三维试验分别检测97株阴沟肠杆菌的AmpC酶,并进行比较。结果 97株受检菌中,75株EDTA纸片法和三维试验均阴性,20株两种方法均阳性,2株菌EDTA纸片法阴性,而三维试验阳性。两种检测方法阳性符合率为90.9%,阴性符合率为100%,总符合率为97.9%。结论 ED-TA纸片法检测阴沟肠杆菌与三维试验的符合率高,且操作简便,较适合于临床实验室应用。Objective To compare EDTA disk test with three-dimensional test in detection of AmpC β-lactamas produced by Enterobacter cloacae clinical isolates. Method 97 clinically isoloted Enterobacter cloacae strains were collected. EDTA disk test and three-dimensional test were used respectively to detect AmpC enzymes. Result Of the 97 stains detected, 75 stains were negative and 20 were positive by both of the two methods, the other two strains were negative by EDTA disk test and positive by three-dimensional test. The positive coincidence rate of the two methods was 90. 9% and negative coincidence rate was 100% ; the totals rate was 97.9%. Conclusion EDTA disk test and three-dimensional test has a high coincidence rate for the detection of AmpC enzymes; EDTA disk test is more suitable for clinical laboratory.

关 键 词：AMPC酶 EDTA纸片法 三维试验 

分 类 号：R378[医药卫生—病原生物学]