A Genetic Pathway for Tapetum Development and Function in Arabidopsis  

作　　者：Jun Zhu Yue Lou Xiaofeng Xu Zhong-Nan Yang 

机构地区：[1]College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

出　　处：《Journal of Integrative Plant Biology》2011年第11期892-900,共9页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (30925007);Shanghai(11ZR1425800);the State Key Basic Research and Development Program of China (2007CB947600)

摘　　要：In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis （programmed cell death）. The tapetum provides many of the proteins, lipids, polysaccharides and other molecules necessary for pollen development. Several transcription factors, including DYT1, TDF1, AMS, MS188 and MS1, have been reported to be essential for tapetum development and function in Arabidopsis thaliana. Here, we present a detailed cytological analysis of knockout mutants for these genes, along with an in situ RNA hybridization experiment and double mutant analysis showing that these transcription factors form a genetic pathway in tapetum development. DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. The genetic pathway revealed in this work facilitates further investigation of the function and molecular mechanisms of tapetum development in Arabidopsis.In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis （programmed cell death）. The tapetum provides many of the proteins, lipids, polysaccharides and other molecules necessary for pollen development. Several transcription factors, including DYT1, TDF1, AMS, MS188 and MS1, have been reported to be essential for tapetum development and function in Arabidopsis thaliana. Here, we present a detailed cytological analysis of knockout mutants for these genes, along with an in situ RNA hybridization experiment and double mutant analysis showing that these transcription factors form a genetic pathway in tapetum development. DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. The genetic pathway revealed in this work facilitates further investigation of the function and molecular mechanisms of tapetum development in Arabidopsis.

关 键 词：anther development genetic pathway pollen development TAPETUM transcription factor DYT1 TDF1 AMS MS188 MS1. 

分 类 号：Q949.776.4[生物学—植物学] Q943