射频消融治疗兔VX-2肾癌后外周血T淋巴细胞亚群的变化  

Effect of radiofrequency ablation on T lymphocyte subsets in peripheral blood of rabbits with renal VX-2 carcinoma

作　　者：张顺[1] 刘光香[1] 王玉忠[1] 郭宏骞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院泌尿外科,210008

出　　处：《中华实验外科杂志》2011年第12期2071-2073,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：江苏省卫生厅课题（Z200712）;南京市卫生局一般项目（YKK08060）;南京市科技发展计划项目（201001104）

摘　　要：目的观察射频消融治疗兔VX-2肾癌后外周血T淋巴细胞亚群变化。方法30只新西兰大白兔肾脏接种VX-2瘤组织建立兔肾癌模型，随机分成A组（射频消融组）、B组（根治性肾切除组）和C组（肿瘤对照组），每组10只。应用流式细胞仪测定治疗前和治疗后28d外周血CD3＋T、CD4＋T、CD8＋T水平的变化。结果射频消融组治疗后28d外周血CD3＋T、CD4＋T、CD4＋／CD8＋水平明显升高，CD8＋T明显下降，与治疗前比较差异有统计学意义（P〈0．05），与根治性肾切除组比较差异有统计学意义（P〈0．05）。根治性肾切除组治疗后28d外周血CD3＋T、CD4＋T、CD4＋／CD8＋水平略有升高，CD8＋T略有下降，与治疗前比较差异无统计学意义（P〉0．05），但与肿瘤对照组比较差异有统计学意义（P〈0．05）。结论射频消融冶疗可引起外周血T淋巴细胞亚群显著变化，也许能增强机体的抗肿瘤细胞免疫功能。Objective To study the effectof radiofrequency ablation （RFA） on T lymphocyte subsets in peripheral blood of rabbits with renal VX-2 carcinoma. Methods Thirty New Zealand rabbits with VX-2 renal carcinoma were equally randomized into 3 groups. RFA and radical nephrectomy were applied in groups A and B respectively. In group C （sham operation）, animals were subjected to laparotomy and abdominal closure without any intervention. The cytokine profiles of T lymphocytes in peripheral blood such as CD3＋ T, CD4＋ T, CD8 ＋T cells were examined by flow cytometry before and 28 days after operation. Results After RFA treatment, the proportion of CD3 ＋ T, CD4 ＋T and the ratio of CD4 ＋/CD8 ＋ were significantly increased （P 〈 0. 05 ） and CD8 ＋ T significantly decreased in group A （ P 〈 0. 05 ） as compared with group B. In group B and group C, there was no significant difference before and after operation. However, there was significant difference in T lymphocyte subsets between groups B and C after operation. Conclusion RFA can result in remarkable changes of T-lymphocyte subsets in peripheral blood, which may also improve the anti-tumor cellular immunity.

关 键 词：射频消融 肾癌 T淋巴细胞 

分 类 号：R737.11[医药卫生—肿瘤]