尿道脱细胞基质和内皮祖细胞的生物相容性  

Biocompatibility of urethral extracellular matrix with endothelial progenitor cells of dogs

作　　者：杨嗣星[1] 赵凯亮[1] 李永伟[1] 宋超[1] 廖文彪[1] 孟令超[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《中华实验外科杂志》2011年第12期2098-2100,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872594）

摘　　要：目的探讨尿道脱细胞基质（UECM）和内皮祖细胞（EPCs）的生物相容性。方法分离培养犬EPCs，以1．0×10^6个／cm2的密度种植于犬UECM上，观察细胞的黏附、生长及增殖。噻唑蓝（MTT）比色法体外检测UECM浸提液（100％、50％、25％、12．5％）对EPCs的细胞毒性。将复合培养物植入8条犬背部皮下检测其组织相容性。结果成功培养犬EPCs，其表面标志表达分别为VEGFR2（82．0±6．5）％，CD34（30．1±4．7）％和CD133（15．9±5．2）％。犬EPCs能够在UECM表面正常黏附、生长、增殖。MTT法检测材料细胞毒性分级为0级或1级。皮下植入实验中动物无异常反应，移植物能在体内降解并引导新生血管长人。结论可以应用EPCs作为种子细胞，UECM作为支架材料构建组织工程化尿道。Objective To evaluate the bioeompatibility of urethral extracellular matrix （UECM） with endothelial progenitor cells （EPCs） of dogs, and discuss the feasibility of the urethra of tissue engineering. Methods EPCs of dogs were isolated and cultured. The biocompatibility of the EPCs and UECM was detected in vitro. The EPCs at a density 1.0 × 10^6/cm2 cocombined with the UECM was cultured and seeded in vitro, of which the adhesion and proliferation were observed. The cytotoxicity of extracts from UECM （ 100%, 50%, 25%, 12. 5% ） was detected by methyl thiazol tetrazolium （MTT） assay, and the histocompatibility was evaluated by implanting the mixture of UECM with EPCs into the back of eight dogs. Results EPCs were isolated and cultured successfully. The cell markers included VEGFR2 （82. 0 ± 6.5）% , CD34 （30. 1 ±4.7）%, CD133 （15.9 ±5.2）%. The UECM was prepared. The co-cuhure of UECM with EPCs indicated that UECM had a good cytocompatibility. The dogs in the implantation test had no abnormal response. The UECM was degraded gradually in vitro, and neovasculature could be seen in the materials after eight weeks. Conclusion EPCs could serve as seeded ceils, and the UECM could serve as scaffold to construct the urethra of tissue engineering.

关 键 词：尿道脱细胞基质 内皮祖细胞 生物相容性 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]