罗非鱼胰岛移植对糖尿病小鼠的治疗作用  

Effectiveness of tilapia islets transplantation in diabetic mice

作　　者：张圣祥[1] 谢燕婷[2] 杨永康[1] 朱哲[1] 周主青[1] 王圣刚[1] 所广军[1] 赵中辛[1] 

机构地区：[1]同济大学附属东方医院普外科,上海200120 [2]同济大学医学院07级实习医生

出　　处：《中华实验外科杂志》2011年第12期2169-2170,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察罗非鱼胰岛腹腔内移植对糖尿病小鼠的治疗作用。方法胶原酶消化分离罗非鱼胰岛。链脲霉素小鼠腹腔内注射，建立药物性糖尿病小鼠模型。消化分离罗非鱼胰岛后，移植入糖尿病小鼠腹腔内，即胰岛移植组（n=14只，简称胰岛组）；设立生理盐水注射（n=10只，简称盐水组）为对照组。比较移植后两组糖尿病小鼠的存活率；动态观察小鼠血糖的变化。并对移植物进行病理检查和免疫组织化学检测。结果移植后1d胰岛组糖尿病小鼠血糖降至正常（5．81±2．73）mmol／L，且持续正常达（3．70±0．95）d，与盐水组（20．73±4．07）mmol／L比较，差异有统计学意义（P〈0．05）。而移植后胰岛组受体存活率与盐水组比较，差异无统计学意义（P〉0．05）。胰岛组移植后3d，移植物周围有大量炎症细胞浸润，罗非鱼胰岛变性坏死。移植后各个时间点在罗非鱼胰岛周围均未发现小鼠IgM、IgG的沉积。结论罗非鱼胰岛腹腔内移植能短暂降低糖尿病小鼠的血糖。以细胞免疫为主的免疫排斥反应是导致移植物死亡的主要原因。Objective Toobserve the effect of tilapia islets transplantation through intraperitoneal injection in diabetic mice. Methods Tilapia islets were harvested, and isolated with the collagenase enzyme. The diabetic models in BALB/C mice were made by intraperitoneal injection of staptozocine （STP）. Diabetic mice were divided randomly into 2 groups： islets group （n = 14）, NS group （n=10）. The survival rate of all groups was observed. The blood glucose levels were determined and compared after transplantation. The samples were evaluated and examined histologically in Hematoxylin and Eosin （HE） and Immunohistochemistrical staining. Results The 3-week survival rate in islets group was higher than in NS group （ P 〈 0. 05 ）. In islets group, tilapia islets could maintain normoglycemia for （3.70 ＋- 0. 95 ） days in diabetic mice. At the 5th day after transplantation, the grafts showed a globally necrosis in islets group. No deposition of IgM and IgG was detected around the grafts at every period in immunohistochemical staining. Conclusion Transplantation of tilapia islets intraperitoneally in diabetic mice could maintain blood glucose levels in a short time. Humoral immunity is not the reason to cause islet early death, but the cell-mediated immune rejection is the reason to cause graft rejection.

关 键 词：胰岛移植 异种 罗非鱼 

分 类 号：R587.1[医药卫生—内分泌]