原花青素处理去细胞猪主动脉瓣细胞相容性研究  

Cytotoxity of acellular porcine aortic valve crosslinked by procyanidins

作　　者：刘洋[1] 刘珊珊[2] 段维勋[1] 朱海龙[1] 金振晓[1] 易定华[1] 刘维永[1] 

机构地区：[1]第四军医大学西京医院心脏外科,西安710032 [2]第四军医大学唐都医院超声诊断科

出　　处：《中华实验外科杂志》2011年第12期2195-2197,共3页Chinese Journal of Experimental Surgery

基　　金：基金资助：陕西省科技攻关计划资助项目（2010K13-02-16）

摘　　要：目的观察原花青素处理去细胞猪主动脉瓣的细胞相容性。方法采用L929细胞经原花青素处理去细胞猪主动脉瓣浸提液培养后，噻唑蓝（MTT）比色法以100％、50％、25％浸提液组培养L929细胞测定其相对增殖率；将L929细胞与原花青素处理去细胞猪主动脉瓣直接接触培养，逐日观察细胞生长状态；将L929细胞种植于原花青素处理的去细胞猪主动脉瓣表面，扫描电镜观察其生长。结果100％、50％、25％浸提液组培养L929细胞相对增殖率（RGR）在87．5％-111．5％之间，与阳性对照组比较差异有统计学意义（P〈0．01），原花青素处理去细胞猪主动脉瓣性质稳定，细胞毒性程度为0—1级。L929细胞与心包材料直接接触培养生长良好，形态学无明显改变。结论原花青素处理去细胞猪主动脉瓣细胞相容性好。Objective To evaluate the cytotoxieity of proeyanidins for acellular porcine aortic valve. Methods L929 cells were cultured with the extracts obtainted from aeellular porcine aortic valve treated with procyanidins. The relative growth rate of L929 cells cultured with 100%, 50% and 25% extracts was measured by methyl thiazol tetrazolium （MTT） . L929 cells were cultured directly with acellular porcine aortic valve treated with procyanidins. The cell morphology was observed, and the cell number was counted. The morphology of L929 cells cultured on the surface of acellular porcine aortic valve treated with procyanidins was observed under the scanning electron microscopy （SEM）. Results The relative growth rate in groups of 100%, 50% and 25% extracts was between 87.5%-111.5%, and there was significant difference between groups of extracts and control group （P 〈0. 01 ）. The property of acellular porcine aortic valve treated with procyanidins was stable and the toxic levels ranged from grade 0 to 1. There were no signs of cytotoxicity induced by acellular porcine aortic valve treated with procyanidins, and the cell morphology was normal. Conclusion Acellular porcine aortic valve treated with procyanidins has good cytocompatibility in vitro.

关 键 词：原花青素 去细胞猪主动脉瓣 生物材料 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]