苦碟子注射液对人肺癌A549细胞增殖与凋亡的影响  

Effects of Kudiezi injection on proliferation and apoptosis of human lung cancer cell line A549

作　　者：何智群[1] 胡卫东[1] 黎春艳[1] 秦毅[1] 吴开松[1] 

机构地区：[1]武汉大学中南医院,430071

出　　处：《中华实验外科杂志》2011年第12期2240-2242,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：湖北省自然科学基金资助项目（2009CBD054）

摘　　要：目的探讨苦碟子注射液对人肺癌A549细胞增殖与凋亡的影响及机制。方法采用噻唑蓝（MTr）比色法、激光共聚焦显微镜（LSM）、流式细胞仪（FCM）及Westernblot技术观察12．5～400．0s／L不同浓度苦碟子注射液对A549细胞的增殖、凋亡、细胞周期及p53基因表达的影响。结果不同浓度KDI对A549细胞增殖均有抑制作用（P〈0．01），50g/L、100g／L、200g／L及400g／L组细胞凋亡率分别为（12．78±0．29）％、（13．46±0．64）％、（20．52±0．73）％和（13．64±0．21）％，均明显高于对照组的（0．89±0．11）％（P〈0．01），G0／G1期细胞百分比分别为（75．15±1．00）％、（77．52±1．09）％、（79．70±1．48）％和（74．55±0．91）％，均明显高于对照组的（66．28±1．91）％（P〈0．01），ASd9细胞p53表达量较正常对照组升高（P〈0．05）。结论苦碟子注射液对人肺癌A549细胞有一定的抑制作用，能够诱导人肺癌A549细胞凋亡，可能与其促p53蛋白表达从而阻滞细胞从G1期转向S期有关。Objective To observe the effect of Kudiezi injection （KDI）on proliferation and apoptosis of human lung cancer cell line A549. Methods After administration of 12. 5-400. 0 g/L KDI for 24- 72 h,the methyl thiazol tetrazolium （MTT） method was used to investigate the inhibitory effect of KDI on A549 cells. Cell apoptosis and cell cycle arrest were investigated by flow cytometry （FCM）. Western blot was used to detect the expression of p53 protein. Results KDI significantly inhibit the growth of A549 cancer cells in dose-dependent manners. The number of G0/G1 stage cell was （75.15 ± 1.00）%, （77.52 ± 1.09） %, （ 79. 70 ± 1.48 ） % and （74. 55 ± 0. 91 ） %, significant higher than constrol group （ 66. 28 ± 1.91 ） % （ P 〈 0.01 ）, and that of p53-expression of A549 cancer cells was higher than it in constrol group （P 〈 0. 05 ）. Conclusion These findings suggest that KDI can inhibit the proliferation of human lung cancer cell line A549 and have obvious dose-effect relationship. The drug inducing people A549 lung cancer cell apoptosis may be relevant to promoting 1953 protein expression and its block cells from G1 to S period.

关 键 词：肺癌 苦碟子注射液 细胞增殖 细胞凋亡 细胞周期 P53 

分 类 号：R285[医药卫生—中药学]