三氧化二砷诱导人横纹肌肉瘤RD细胞株凋亡的实验研究  

EXPERIMENTAL STUDIES OF APOPTOSIS INDUCED BY ARSENIC TRIOXIDE IN HUMAN EMBRYONAL RHABDOMYOSARCOMA CELL LINE RD

作　　者：龙兴震[1] 张信江[2] 

机构地区：[1]贵阳中医学院第一附属医院皮肤科,贵州550001 [2]遵义医学院第一附属医院皮肤科

出　　处：《现代预防医学》2011年第23期4945-4947,4952,共4页Modern Preventive Medicine

摘　　要：[目的]研究三氧化二砷(As2O3)体外抑制人胚胎型横纹肌肉瘤细胞株RD(RD细胞株)增殖及诱导凋亡的作用。[方法]以不同浓度的As2O3处理RD细胞株,用四甲基偶氮唑蓝(MTT)法测定细胞增殖情况,流式细胞仪检测细胞的DNA含量及凋亡率。[结果]4～8μmol/L As2O3能以时间剂量依赖的方式抑制RD细胞株的增殖,并诱导其凋亡,在流式细胞仪DNA线条图上出现典型的亚二倍体"凋亡峰",细胞周期阻滞于G0/G1期,S期和G2/M期细胞减少;低浓度As2O3(﹤2μmol/L)则有轻微促增殖作用,细胞周期向S期和G2/M期集中,G0/G1期细胞减少。[结论]As2O3浓度≥4μmol/L时,可明显抑制RD细胞株的生长并促进细胞凋亡,其临床应用还需进一步研究。[Objective]To investigate the effect of a wide range of concentrations of arsenic trioxide（As2O3）on human embryonal rhabdomyosarcoma cell line RD in vitro.[Methods]RD cell line was dealt with As2O3 at different concentrations.Cell proliferation was evaluated by the MTT assay,and the apoptosis and cell cycles changes of RD cell line induced by arsenic trioxide were measured by flow cytometry methods.[Results]It was found that 4-8μmol/L of As2O3 obviously inhibited the proliferation of human embryonal rhabdomyosarcoma cell line RD in time-dependent and dose-dependent pattern.Percentages of apoptosis and G0/G1 phase cell were increased,S and G2/M phase cells were decreased,and a typical hypodiploid apoptosis peak in the DNA line was found in the flow cytometry with the treatment of As2O3 at 4-8 μmol/L.When the concentration of As2O3 was lower than 2μmol/L,the growth of RD was enhanced lightly,in the meantime,the percentages of S and G2/M phase cell were increased and G0/G1 phase cells were decreased.[Conclusion]As2O3（≥4μmol/L）can obviously depress the proliferation of RD and accelerate the apoptosis in vitro.

关 键 词：横纹肌肉瘤 三氧化二砷 细胞培养 凋亡 流式细胞术 

分 类 号：R979.1[医药卫生—药品]