实时荧光PCR技术检测高危型HPV的临床应用评价  

EVALUATION OF THE CLINICAL PERFORMANCE OF REAL TIME FLUORESCENCE PCR TEST FOR DETECTION OF HIGH RISK HPV

作　　者：崔金环[1] 杨光[1] 苏锡康[1] 薛红燕[1] 李晓杰[1] 曾劲伟[1] 林爱珍[1] 

机构地区：[1]广东省佛山市第一人民医院,佛山52800

出　　处：《现代预防医学》2011年第23期4948-4949,4952,共3页Modern Preventive Medicine

摘　　要：[目的]评价实时荧光PCR技术检测高危型HPV的临床应用效果。[方法]应用实时荧光PCR技术检测80例已经HCⅡ方法检测高危型HPV的宫颈脱落细胞标本,其中40例为阴性标本;40例为阳性标本,比较两种方法检测结果的一致性。结果不一致的应用HPV基因分型试剂进行检测。以病理学检查结果作为金标准,评价实时荧光PCR技术的临床应用效果。[结果]80例标本的实时荧光PCR检测结果中,76例的结果与HCⅡ结果一致,总符合率为92.50%,Kappa值为0.85。6例结果不一致的标本,经HPV分型检测有1例为HPV6型,其余5例均为阴性。实时荧光PCR技术检测的灵敏度、特异度、阳性预测值和阴性预测值分别为93.33%,63.08%,39.47%和97.62%。[结论]实时荧光PCR技术与HC-Ⅱ方法检测结果一致性高,是检测高危型HPV感染的另一有效手段,可在临床上用于宫颈癌及其癌前病变的筛查。[Objective]To evaluate the clinical performance of real-time fluorescence PCR test for detection of high risk HPV.[Methods]80 of cervical cytology specimens were detected by real time fluorescence PCR test.These cases had detected by Hybrid CaptureⅡ（HCⅡ）,40 were positive and 40 were negative results.The accordance of two methods was compared.The conflicting samples were detected by HPV genotype.Pathological diagnosis was used as golden standard,the clinical performance values of real-time fluorescence PCR were assessed.[Results]In 80 cases,76 were accordant by real time fluorescence PCR and HCⅡ.The accordance of the two methods was 92.50%,Kappa value was 0.85.In 6 of conflicting samples,1 was HPV 6 and 5 were negative by HPV genotypes.The sensitivity,specificity and positive and negative predictive values of real-time fluorescence PCR was 93.33%,63.08%,39.47% and 97.62%,respectively.[Conclusion]Real time fluorescence PCR has good accordance with HCⅡ,it is another effective method of detecting high risk HPV for screening cervical cancer and precancerous lesions.

关 键 词：实时荧光PCR 杂交捕获二代法 高危型人乳头瘤病毒 

分 类 号：R378.3[医药卫生—病原生物学]