应用绿色荧光蛋白标记迟缓爱德华菌感染斑马鱼  

Visualisation of zebrafish infection with GFP-labelled Edwardsiella tarda

作　　者：王雪鹏[1] 丁雷[1] 邹兰柱 陈少波[3] 谢起浪[3] 闫茂仓[3] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]泰安市水产研究所,泰安271000 [3]浙江省海洋水产养殖研究所,温州325000

出　　处：《中国实验动物学报》2011年第5期433-436,I0007,I0008,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：山东省优秀中青年科学家科研奖励基金(BS2011HZ012);中国博士后基金(2011490162);浙江省近岸水域生物资源开发与保护重点实验室开放基金(2010F30003);浙江省科技厅创新团队建设与人才培养项目(2009F20009)

摘　　要：目的建立斑马鱼模型研究迟缓爱德华菌的致病性及感染途径。方法应用绿色荧光蛋白标记迟缓爱德华菌,追踪观察其感染斑马鱼的动力学过程及病理组织学变化。结果病理组织学检查以肝脏水肿变性,肝细胞萎缩、坏死、脱落,脾脏散在增生性结节、充血、水肿、淋巴细胞大量缺失等病变为主;感染后,该菌先后在斑马鱼肠道、鳃和皮肤中定植。结论斑马鱼可作为研究迟缓爱德华菌致病性的动物模型。肠道、鳃和皮肤可能是迟缓爱德华菌先后感染斑马鱼的主要途径。Objective To establish a zebrafish model of Edwardsiella tarda infection to study the pathogenicity and possible route of infection.Methods The infection process and histopathology were visualized in the zebrafish infected by E.tarda labelled with green fluorescent protein.Results Histopathological observation revealed hydropic degeneration,atrophy and necrosis of liver cells,scattered hyperplastic nodules,congestion,edema and loss of lymphocytes in the spleen.The gastrointestinal tract was the first site where the pathogen was detected,followed by gill and skin.Conclusions Zebrafish can be used as an animal model for studying the pathogenicity of E.tarda infection.The results of our study provide evidence that intestine,gill and skin may be the main route of infection by E.tarda.

关 键 词：迟缓爱德华菌 绿色荧光蛋白 斑马鱼 致病性 感染 

分 类 号：Q95-33[生物学—动物学] S-94[农业科学]