IL-1β对RAW264.7细胞表达IL-4mRNA,GATA-3mRNA的影响  

Effects of IL-1β on Expressions of IL-4 and GATA-3mRNA of RAW264.7 Cells

作　　者：庄伟[1] 牟东珍[1] 林志娟[1] 孙萍[1] 祝仁军[1] 梁淑娟[1] 

机构地区：[1]潍坊医学院免疫学教研室,山东省高校免疫学重点实验室山东潍坊261053

出　　处：《潍坊医学院学报》2011年第2期85-87,共3页Acta Academiae Medicinae Weifang

基　　金：潍坊市科学技术发展计划（课题编号：200803068）

摘　　要：目的 探讨IL-1β对RAW264.7细胞表达IL-4,GATA-3mRNA的影响.方法 常规培养RAW264.7细胞后进行干预,A组：IL-1β（0mg/L,1mg/L,10mg/L,20mg/L）刺激细胞1h,B组：IL-1β（10mg/L）刺激0h,30min,1h,3h,6h,C组：IL-1β（10mg/L）刺激0h,30min,1h,1.5h,2h,采用反转录聚合酶链反应（RT-PCR）分别检测IL-4,GATA-3mRNA的表达.结果 IL-Iβ刺激RAW264.7细胞不同浓度、不同时间与空白对照组比较IL-4,GATA-3mRNA的表达增高（P＜0.05）;IL-1β（10mg/L）刺激RAW264.7细胞1h时,IL-4mRNA的表达量最高,1.5h时GATA-3mRNA的表达量最高.结论 IL-1β在RAW264.7细胞中促进IL-4,GATA-3mRNA的表达呈浓度、时间依赖性,提示IL-1β通过促进单核巨噬细胞分泌IL-4,GATA-3,诱导Th2型细胞分化,促进和加重哮喘的慢性炎症.Objective To observe the effect of IL-1β on expressions of IL-4,GATA-3mRNA of RAW264.7 cells. Methods The cultured cells of the fourth to fifth passage were used for experiments. 2 ×10^5/ml cells were seeded in 24-well plates,when cells reached 80% filsion,the following treatment would he clone, group A ： cells were treated with IL-1β （ 0mg/L, 1 mg/L, 10mg/L, 20mg/L） for 1 h, group B ： cells were treated with IL-1β（10mg/L） for 0h,30min,lh,3h,6h,group C：cells were treated with IL-IiS（10rag/L） for 0h,30min,1h,1.5h, 2h. Expressiun of IL-4 and GATA-3mRNA in group A,expression of IL-4mRNA in group B and expression of GATA-3mRNA in group C were detected by reverse transcription polymerase chain reaction（ RT-PCR）. Results Increase of IL-4 and GATA-3mRNA expression was found when RAW264.7 cells were stimulated with different concentrations and different time compared with the control group （ P 〈 0.05 ）. When RAW264.7 cells were treated with IL-1β（10mg/L） for 1 h, expression of IL-4mRNA reached the highest level;When treated for 1.5h,expression level of GATA-3mRNA was the highest. Conclusion IL-113 induces IL-4 and GATA-3mRNA expression iu a time-dependent and concentration-dependent manner in RAW264.7 cells,which suggests that IL-1β induces the monocyte macrophages production of IL-4 and GA- TA-3,induces differentiation of Th2 type cells,and plays an important role in promoting and aggravating chronic inflammation of asthma.

关 键 词：IL-1Β IL-4 GATA-3 RAW264.7细胞 

分 类 号：R392.12[医药卫生—免疫学]