阻断CD40-CD40配体共刺激信号诱导的移植免疫耐受对树突状细胞的影响  

The impact of transplant tolerance induced by CD40-CD40L(Ligand) costimulation blockade on dendritic cells(DCs)

作　　者：姜晓峰[1] 郭大伟[1] 崔哲铭[1] 朱磊[1] 孙文郁[1] 林琳[1] 唐裕福[1] 王学范[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院普外科,沈阳110032

出　　处：《中国免疫学杂志》2011年第9期800-803,共4页Chinese Journal of Immunology

基　　金：教育部留学回国人员科研启动基金资助项目(No.2008890);辽宁省教育厅高等学校科研项目计划(No.2008824);中国医科大学附属第四医院院内课题项目(No.2006J06)

摘　　要：目的:探讨阻断CD40-CD40配体共刺激信号诱导移植免疫耐受对树突状细胞功能的影响。方法:将抗CD40配体抗体(MR-1)应用于小鼠心脏移植受体以诱导移植免疫耐受,使用磁珠细胞提取装置从排斥及免疫耐受受体中提取树突状细胞。使用流式细胞仪测定树突状细胞表面分子CD40、CD80及CD86的表达。于体外以LPS再刺激树突状细胞,并使用ELISA法测定上清中的细胞因子IL-10和IL-12的水平。同时采用混合淋巴细胞反应(MLR)测定了树突状细胞的刺激性及免疫调节能力。结果:免疫耐受受体的树突状细胞表达低水平的共刺激分子CD40、CD80及CD86,同时分泌高水平的IL-10和低水平的IL-12。而且这些树突状细胞具有较弱的刺激性,并能抑制脾细胞的增殖。结论:阻断CD40-CD40配体共刺激信号可以产生免疫耐受状态,在这样的免疫耐受受体中诱导出未成熟表型的IL-10highIL-12low树突状细胞,这些细胞具有免疫调节活性。Objective：To investigate the effect of transplant tolerance induced by CD40-CD40L costimulation blockade on the function of DCs.Methods：The mouse recipients with heterotopic heart transplantation were administrated with anti-CD40L mAb（MR-1） to induced the transplant tolerance.DCs were sorted from rejected and tolerant recipients using MACS.Their expression of CD40,CD80 and CD86 was examined using FACS.DCs were stimulated with LPS in vitro and IL-10,IL-12 levels in the supernatants were evaluated using ELISA.We also investigated their stimulatory capability and tolerogenic capability using mixed leukocyte reaction（MLR）.Results：DCs from tolerant recipients expressed lower CD40,CD80,CD86,and secreted higher level of IL-10 and lower level of IL-12.Furthermore,DCs from tolerant recipients were weak stimulators of the MLR and inhibited the proliferation of splenocytes.Conclusion：IL-10highIL-12low DCs with immature phenotype were induced in the transplant tolerance with treatment of CD40-CD40L costimulation blockade and they obtained the tolerogenic function.

关 键 词：树突状细胞 未成熟 免疫调节活性 移植免疫耐受 

分 类 号：R392.4[医药卫生—免疫学]