ω-3鱼油脂肪乳对博莱霉素致肺纤维化大鼠的干预作用  

The effects of ω-3 fat emulsion on bleomycin-induced pulmonary fibrosis in rats

作　　者：邓超[1] 曹玉芳[1] 陈栩栩[1] 殷宗宝[1] 

机构地区：[1]海南省海口市人民医院重症医学科,570208

出　　处：《广东医学》2011年第18期2370-2372,共3页Guangdong Medical Journal

摘　　要：目的探讨ω-3鱼油脂肪乳对博莱霉素致肺纤维化大鼠的干预作用及其机制。方法将清洁级雄性大鼠90只随机分成生理盐水对照组(对照组)、博莱霉素所致大鼠肺纤维化模型组(模型组)和博莱霉素所致大鼠肺纤维化+ω-3鱼油脂肪乳干预组(干预组)。在第7、14、21天分别取左肺下叶供免疫组化检测转化生长因子β1(TGF-β1)、γ干扰素(IFN-γ)表达,右肺下叶供HE染色病理观察;用ELISA双抗体夹心法测定各组大鼠血清白细胞介素-4(IL-4)和IFN-γ含量。结果 (1)干预组大鼠肺泡炎、肺纤维化程度均较轻;(2)模型组大鼠在第7、14、28天的肺组织TGF-β1染色呈强阳性,IFN-γ染色较淡;而干预组TGF-β1、IFN-γ染色及定量表达则相反(P<0.01),且第7、14、28天TGF-β1定量表达逐渐减少,IFN-γ逐渐增加(P<0.05)。(3)模型组大鼠第7、14、28天血清IL-4含量较高,IFN-γ含量较低;而干预组则相反(P<0.01),且第7、14、28天IL-4含量逐渐降低,IFN-γ含量逐渐升高(P<0.05)。结论ω-3鱼油脂肪乳可以下调大鼠肺组织TGF-β1的表达,降低其血清含量和IL-4含量;上调肺组织IFN-γ的表达,增加其血清含量,从而减轻肺纤维化。Objective To evaluate the effects of ω - 3 fat emulsion on bleomycin - induced pulmonary fibrosis by bleomycin in rats. Methods Ninety rats were randomly divided into 3 groups： NS group （control group）, pulmonary fibrosis group （model group） and to -3 fat emulsion treatment group （treatment group）. On 7th, 14th and 21st day, the left lung tissues were obtained for assessment of TGF - β1 and IFN -γ expression with immunohistometry. The right lung tissues were obtained for HE staining. Meanwhile, serum IFN - γ and IL - 4 were assessed with ELISA. Results （ 1 ） Significantly milder pulmonary alveolitis and fibrosis were revealed in treatment group than those in model group. （2） Strong positive TGF - β1 protein expression with weakly positive IFN -γ was detected on bronchiolar epithelium in model group. However, the significantly opposite expression was revealed in treatment group （P 〈 0. 01 ）. Meanwhile, on the 7th, 14th and 21st day, significantly down- regulation and up- regulation of TGF-β1 and IFN -γ, respectively, were observed in the treatment group following time - dependent manners （ P 〈 O. 05 ）. （3） Significantly higher IL - 4 and low- er IFN -γ were revealed in model group than those in treatment group （P 〈0. 01 ）. Meanwhile, on the 7th, 14th and 21st day, significantly down - regulation and up - regulation of IL - 4 and IFN - γ, respectively, were observed in the treatment group following time - dependent manners （P 〈 0. 05）. Conclusion The to - 3 fat emulsion can alleviate pulmona- ry fibrosis via down - regulation of TGF - β1 protein and IL - 4 levels and up - regulation of IFN - γ, protein.

关 键 词：肺纤维化 博莱霉素 Γ干扰素 Ω-3鱼油脂肪乳 转化生长因子Β1 白细胞介素-4 

分 类 号：R285.5[医药卫生—中药学]