贵州省及部分周边地区牛博尔纳病病毒感染的情况调查  

Borna disease virus in blood of cattle infect detection in Gui Zhou and surrounding areas

作　　者：刘海军[1] 王长明[1] 谢婷婷[1] 王顺旺[1] 徐平[1] 

机构地区：[1]遵义医学院附属医院神经内科,贵州遵义563003

出　　处：《重庆医学》2011年第32期3296-3299,共4页Chongqing medicine

基　　金：贵州省优秀青年科技人才基金资助项目(2007-15)

摘　　要：目的探讨贵州省及周边地区牛群博尔纳病病毒(BDV)感染状况。方法采用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)检测了120例牛的外周血单个核细胞中的BDV p24基因片段。结果牛外周血单个核细胞(PBMCs)中BDVp24基因片段阳性率均为4.17%。牛BDV p24基因片段的测序结果与GeneBank提供的标准病毒株比较,同源性96.51%～97.67%;有2个位点出现一致性沉寂突变(nt1675C-T,nt1678T-C突变率为2%);与马的Strain V病毒株比较,有3个位点出现一致性沉寂突变(nt1650T-C,nt1671C-T,nt1674C-T突变率为3%);与H1766病毒株比较,有2个位点出现一致性沉寂突变(nt1675C-T,nt1678T-C突变率为2%);与He80/FR病毒株比较,在3个位点出现一致性沉寂突变(nt 1660T-C,nt 1669A-G,nt 1672C-T突变率为3%);但它们所编码的氨基酸没有改变。结论贵州及湖南省部分地区牛群中存在BDV自然感染,可能是BDV流行区域之一;人感染BDV可能具有潜在的动物源性。Objective To investigate the infection condition in cattle infected with Borna disease virus（BDV）in Guizhou province and some surrounding areas.Methods The p24 gene fragment of BDV-RNA in peripheral blood mononuclear cells（PBMCs）from 120 cattle was detected by fluorescence quantitative nested reverse transcriptase polymerase chain reaction（FQ-nRT-PCR）.Results The p24 gene fragment of BDV-RNA in PBMCs from cattle was 5 positive（4.17%）.The homology of p24 gene fragment of BDV detected to GenBank was 96.51%-97.67%.2 situs consistency silent mutation when compared with Bo/04W and huP2br isolated in heifer brain tissue by Watanabe Y（nt1675 C-T,nt1678 T-C mutation rate of 2%）.3 situs consistency silent mutation when compared with Strain V in horse（nt1650 T-C,nt1671 C-T,nt1674 C-T mutation rate of 3%）.2 situs consistency silent mutation when compared with H1766 in horse（nt1675 C-T,nt1678 T-C mutation rate of 2%）.3 situs consistency silent mutation when compared with He80/FR（nt 1660 T-C,nt 1669 A-G,nt 1672 C-T mutation rate of 3%）.However,there was no change in the encoding amino acid residues.Conclusion There is BDV natural infection probably originated from cattle of Gui Zhou and surrounding areas,also probably is one of the prevalence regions of BDV.BDV might play a potential role in the development of human having correlations with animals.

关 键 词：Born病病毒 荧光定量巢式逆转录聚合酶链反应 外周血单个核细胞 

分 类 号：S858.23[农业科学—临床兽医学]