拟南芥β-罗勒烯合成酶基因T-DNA插入突变体的鉴定  

Identification of T-DNA Insertion Homozygous Mutant Lines for Analyzing β-ocimene Synthase in Arabidopsis thaliana

作　　者：马泉芳[1] 魏然[1] 刘春林[1] 

机构地区：[1]湖南农业大学农学院,长沙410128

出　　处：《作物研究》2011年第5期477-481,共5页Crop Research

基　　金：国家自然科学基金重点项目(31071455)

摘　　要：β-罗勒烯(β-Ocimene)是一种单萜挥发性有机物,亦是一种重要的植物通讯信号分子。为了进一步研究β-罗勒烯的作用机理,需要获得β-罗勒烯合成酶基因表达沉默的实验材料。根据TAIR网站上公布的β-罗勒烯合成酶信息,从美国拟南芥生物资源中心购买该基因的T-DNA插入突变体材料,运用双引物法对这些突变体进行了T-DNA插入位点的鉴定,获得8株稳定遗传的纯合突变体,该结果为深入研究β-罗勒烯的功能奠定了良好基础。β-ocimene is one of monoterpene,an important plant communication signaling molecule.In order to further study the role of β-ocimene mechanism,homozygous mutant with β-ocimene synthase gene expression silence must be got.According to the information published on the TAIR website,we bought the T-DNA insertion mutant of the β-ocimene synthase gene from the TAIR.These homozygous mutants were identified by the way of double-primer method.Finally,8 stable homozygous genetic mutants were obtained.These homozygous mutants lay good foundation for further investigating the function of β-ocimene.

关 键 词：β-罗勒烯 单萜合酶AtTPS03 T-DNA插入突变 拟南芥 

分 类 号：Q78[生物学—分子生物学]