山东地区猪繁殖与呼吸综合征病毒分子特征  

Molecular Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in Shandong Province

作　　者：战翔 李娟 张熙艳 刘洋[2] 李俊[2] 

机构地区：[1]济南市畜牧兽医局疫控中心,山东济南250000 [2]山东省农业科学院畜牧兽医研究所,山东济南250100

出　　处：《家畜生态学报》2011年第5期26-30,共5页Journal of Domestic Animal Ecology

基　　金：兽医生物技术国家重点实验室开放基金课题(SKLVBF201007);山东省自然基金项目(ZR2010CQ003)

摘　　要：为揭示2009~2010年山东地区流行的猪繁殖与呼吸综合征病毒（PRRSV）的分子特征,通过RT-PCR扩增采自山东地区不同猪场病猪组织样本中PRRSV的Nsp2、ORF5、ORF6、ORF7序列,用DNAStar软件分析所测序列,并与北美洲型VR-2332株、欧洲型LV株及国内分离株进行核苷酸和推导的氨基酸同源性比较。结果表明,Nsp2、ORF5、ORF6、ORF7核苷酸与北美洲型VR-2332的同源性分别为77%~79.9%、88.3%~89.6%、94.9%~95.8%、93.3%~94.1%,与欧洲型LV株的同源性分别为29.6%~31.6%2、64.1%~64.8%、68.3%~68.6%、66.2%~67.3%,证明山东地区的PRRSV毒株仍属北美洲型。系统进化分析表明它们与国内2006~2010年间的分离株（变异株）亲缘关系较近,而与更早的分离株和疫苗MLV株亲缘关系较远,未发现传统毒株。在Nsp2、ORF5、ORF6、ORF7基因上均有不同程度的点突变。本研究揭示了山东地区流行的PRRSV各基因的变异特征,为PRRSV的综合防控提供理论依据。In order to reveal the molecular characteristics of porcine reproductive and respiratory syndrome virus from the year of 2009~2010 in Shandong region,the Nsp2,ORF5,ORF6 and ORF7 gene of PRRSV was amplified with RT-PCR from samples of pigs in Shandong.Compared to those of VR-2332,LV and other strains isolated in China,the Nsp2,ORF5,ORF6 and ORF7 genes shared 77.0%~79.9%,88.3%~89.6%,94.9%~95.8% and 93.3%~94.1% nucleotide homologies with the North American type and shared 29.6%~31.6%,64.1%~64.8%,68.3%~68.6% and 66.2%~67.3% with European LV strain.This indicated that the isolated strains were related to the genotype of North American PRRSV.The phylogenetic analysis indicated that the prevalent PRRSV in Shandong from 2009~2010 had closer genetic relationship with those isolates from other areas of China than the earlier field isolates and the vaccine strain MLV,and there was no traditional strains.There were point mutations in Nsp2,ORF5,ORF6 and ORF7 genes.This study revealed the characteristic variations of prevalent PRRSV in Shandong and provided a theoretical basis for controlling this disease.

关 键 词：PRRSV NSP2基因 ORF5基因 ORF6基因 ORF7基因 序列分析 

分 类 号：S811.5[农业科学—畜牧学]