苦瓜籽蛋白的分离纯化及免疫原性分析  

Isolation,Purification and Characterization of RIP from Seeds of Momordica charantia

作　　者：杨梅强[1] 聂宇[1] 姚兴川[1] 王殊睿[1] 刘敏[1] 赵欣平[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室动物疾病预防与食品安全四川省重点实验室,成都610064

出　　处：《四川动物》2011年第6期893-896,共4页Sichuan Journal of Zoology

摘　　要：经过NaCl溶液匀浆抽提、硫酸铵分级分离、SP-SepharoseFastFlow层析、SephacrylS-100层析、Macro-Cap-SP层析,从苦瓜籽中同时分离纯化出大量的α-苦瓜素和苦瓜抗HIV蛋白30。经SDS-PAGE鉴定,二者均为单一条带,表观分子量为28kDa。用两种蛋白分别免疫Balb/c小鼠,双向免疫扩散表明,两种苦瓜籽蛋白无交叉反应。本实验为进一步探索PEG修饰MAP30免疫原性变化打下了基础。Large amounts of α-momorcharin（α-MMC） and MAP30 were quickly and easily isolated and purified from the seeds of Momordica charantia by following procedures：extraction with NaCl solution,ammonium sulfate fractionation,chromatography on SP-Sepharose Fast Flow,chromatography on Sephacryl S-100,and chromatography on Macro-Cap-SP.The results of SDS-PAGE showed that the two RIPs were a homogeneous single band,and molecular mass was estimated to be about 28 kDa.Double immunodiffusion showed that the two proteins had no cross reaction.

关 键 词：α-苦瓜素 苦瓜抗HIV蛋白30 分离纯化 双向免疫扩散 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]