电针不同穴位对肠易激综合征模型大鼠内脏敏感性及肠系膜微循环的影响  

Effect of Electro-acupuncture(EA) Different Acupoints on Irritable Bowel Syndrome with Visceral Sensitivity in Rats and Intestinal Microcirculation

作　　者：杨磊[1] 李滢 赵雅芳[1] 孙洁[1] 杨帅[1] 陈磊[1] 朱文莲[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸推拿学院 [2]北京小汤山医院

出　　处：《微循环学杂志》2011年第4期4-6,I0002,共4页Chinese Journal of Microcirculation

基　　金：北京农学院北京市重点实验室开放性课题(No.TCVM-201101);北京中医药大学研究生自主课题(No.2011JYBZZ-XS045)

摘　　要：目的:观察电针天枢、足三里穴对肠易激综合征(IBS)模型大鼠内脏敏感性及肠微循环的影响。探讨针刺治疗IBS的部分机制。方法:将Wistar幼鼠分为正常组、模型组、天枢组、足三里组,每组6只。天枢组和足三里组从实验第6周开始电针介入,隔日1次,共7次。模型组只束缚不针刺,正常组不作任何处理。治疗结束第二天采用XW-B-3冷光源微循环显微仪观察各组大鼠肠系膜微循环不同时点管径和血流速度的变化。结果:(1)内脏敏感性评估:与正常组比较,模型组大鼠腹部抬起、背部拱起值明显降低(P<0.01),与模型组比较,电针天枢、足三里组大鼠腹部抬起、背部拱起值明显升高(P<0.01)。(2)肠系膜微循环检测:①血管直径:与正常组比较,模型组大鼠肠系膜微血管明显收缩(P<0.05);与模型组比较,电针天枢组、足三里组大鼠肠系膜微血管明显扩张(P<0.05或P<0.01);②血流状态:与正常组比较,模型组大鼠肠系膜微循环血流速度明显减慢或停止(P<0.01)。结论:针刺天枢、足三里可明显提高IBS大鼠内脏敏感性阈值,降低IBS大鼠内脏敏感性;明显改善肠系膜微循环血流状态和血管痉挛,从而缓解肠黏膜缺血、缺氧,缓解腹部疼痛。Objective：To observe effects of EA Tianshu point,Zusanli point on the irritable bowel syndrome rat models to discuss a part of the mechanism of IBS＇s acu-puncture treatment.Method：To divide the young VISTAR rats into normal group,model group,Tianshu group and Zusanli group,six per group.The Tianshu group and Zusanli group were started to be given EA in the 6th week,once two days,a total of seven times.Model group was bounded but not given EA.The normal group was done nothing.To use XW-B-3 cold lightmicro-circulation tester to observe the rats＇ state of caps and blood flow of mesentery microcirculation in each group and different time period.Results：（1）The estimate of the visceral sensitivity：compared with the normal group,the value of the model group rats＇ belly raising and back hunch-up significantly reduced（P0.01）.Compared with the model group,the value of the EA Tianshu and Zusanli group rats＇ belly raising and back hunch-up significantly increased（P0.01）.（2）Test of the mesentery microcirculation：①The diameter of the blood vessel：compared with the normal group,the diameter of the mesentery micro-vessels of the model group significantly narrowed（P0.05）;Compared to the model group,the diameter of the mesentery micro-vessels of the EA Tianshu group and Zusanli group significantly expanded（P0.05 or P0.01）.②The state of the blood flow：compared with the normal group,the model group＇s blood flow speed of mesentery micro-vessels significantly reduced or stopped（P0.01）.Conclusion：EA Tianshu,Zusanli can significantly increase the value of the visceral sensitivity threshold of the IBS rats and reduce the visceral sensitivity;it can also significantly improve the blood state of the mesentery microcirculation and remove spasm to relieve the lack of blood and oxygen in the intestinal mucosa.As a result,it has the therapeutic effect of pain relief.

关 键 词：肠易激综合征 微循环 内脏敏感性 电针穴位 大鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]