慢性乙肝患者血清IL-27水平检测及意义  

Detection of Serum IL-27 in the Chronic Patients with HBV Infection and Its Clinical Significance

作　　者：汪付兵[1] 李瑞炎[1] 

机构地区：[1]武汉大学中南医院检验科,武汉430071

出　　处：《微循环学杂志》2011年第4期37-38,42,I0003,共4页Chinese Journal of Microcirculation

摘　　要：目的:初步探讨慢性乙肝(CHB)患者血清白介素-27(IL-27)表达水平及意义。方法:根据临床表现将33例CHB患者分为轻度组(12例)、中度组(11例)和重度组(10例),分别检测各组血清IL-27水平以及谷丙转氨酶(ALT)和γ-谷氨酰转肽酶(GGT)水平;又根据血清乙型肝炎病DNA(HBV-DNA)载量,将患者分成HBV-DNA低载量组(≤105拷贝数/ml)和高载量组(>105拷贝数/ml),比较两组IL-27水平差异。结果:CHB各组血清IL-27水平较健康对照组明显升高(P<0.05),且重度组显著高于轻度组和中度组(P<0.05);不同HBV-DNA载量组血清IL-27水平有显著差异,低载量组显著高于高载量组(P<0.05);CHB患者血清IL-27水平与ALT和GGT水平均呈显著正相关(r分别为0.723和0.664,P<0.01)。结论:IL-27水平升高与CHB严重程度有关;IL-27在清除患者HBV感染的同时也造成其肝细胞炎性损伤。Objective：To investigate the expression level of IL-27 in serum of the patients with chronic hepatitis（CHB） and its clinical significance.Method：IL-27 level was detected by the ELISA method in all patients,at the same time,ALT/GGT level was detected.HBV-DNA level was detected by the fluorescence quantitative PCR method,according to HBV-DNA load,the patients with CHB were divided into low（≤105copies/ml）,and high（105copies/ml） virus load groups.Results：IL-27 level in patients with severe CHB was significantly higher（P0.05） than in moderate and mild groups;the level of IL-27 in all patients with CHB was significantly higher than those in control group;IL-27 level in different virus load groups were significantly different（P0.05）：the level of IL-27 in low virus load groups was significantly higher than in high virus load groups.The level of IL-27 in serum of patients with CHB were positively correlated with level of ALT/GGT in serum（r=0.723,P0.01;r=0.664,P0.01）.Conclusion：IL-27 may participate in hepatic inflammatory injury process during which IL-27 induces the hepatic cells lesion as it clearing HBV,the serum level of IL-27 may be used as the degree of liver damage for patients with CHB.

关 键 词：慢性乙肝 白细胞介素-27 乙型肝炎病毒 

分 类 号：R512.62[医药卫生—内科学]