蒙药章古-3汤对腺嘌呤致大鼠慢性肾衰模型的影响作用研究  

THE THERAPEUTIC EFFECTS OF TRADITIONALMONGOLIAN MEDICINE Zhanggu-Ⅲ ON ADENINE-INDUCEDCHRONIC RENAL FAILURE IN RAT

作　　者：马月宏[1] 爱民[1] 马丽杰[1] 许丽萍[1] 伊乐[2] 

机构地区：[1]内蒙古医学院基础医学院药理教研室,内蒙古呼和浩特010050 [2]内蒙古医学院临床医学院

出　　处：《内蒙古医学院学报》2011年第5期397-400,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古医学院青年基金(NY2008QN012)

摘　　要：目的:研究蒙药章古-3汤对腺嘌呤诱导大鼠CRF的干预作用及其机制。方法:60只Wister大鼠随机分为6组:空白对照组、病理模型组、章古-3汤高剂量治疗组(30g/kg.d)、章古-3汤中剂量治疗组(20g/kg.d)、章古-3汤低剂量治疗组(10g/kg.d)、尿毒清对照组,每组10只。以腺嘌呤200mg/kg.d灌胃建立CRF模型,造模同时各治疗组灌胃给予章古-3汤,尿毒清组给予尿毒清溶液10g/kg.d。连续灌胃24d后,检测生化指标(BUN、Scr、Na+、K+、Ca2+、P5+);观察肾组织病理改变;用免疫组化方法检测肾组织中TGF-β1的表达和分布。结果:经章古-3汤治疗的各组大鼠肾功能与同期病理模型组比较,有明显的改善(P<0.05),与尿毒清对照组相比无显著性差异。治疗组TGF-β1水平显著低于同期病理模型组(P<0.05),但皆高于正常对照组(P<0.01)。结论:蒙药章古-3汤对腺嘌呤导致CRF大鼠模型的肾功能有一定保护作用,其作用机制与抑制TGF-β1表达有关。Objective：To study the therapeutic effects of Traditional Mongolian Medicine Zhanggu-Ⅲ on adenine-induced chronic renal failure（CRF） in rat and explore the mechanism of Zhanggu-Ⅲ in preventing and treating CRF.Methods： Sixty wister rats were randomly divived into six groups：normal group,model group,Zhanggu-Ⅲ high dosage group（ZG-ⅢH）,Zhanggu-Ⅲ middle dosage group（ZG-ⅢM）,Zhanggu-Ⅲ low dosage group（ZG-ⅢL）,and Niaoduqing group.each group had 10 rats.Each rat in model group,ZG-Ⅲ groups and niaoduqing group was infused with Adenine（20g/Kg·d） into stomach.in the same time,each rat in ZG-Ⅲgroup was also infused with high（30g/kg·d）,middle（20g/kg·d）,low（10g/kg·d） dosage forms ZG-Ⅲ into stomach for 24 days;the Niaoduqing group was given Niaoduqing liquid.The content of blood urea nitrogen（BUN）,serum creatinine（Scr）,biochemical indexes（Ca^2＋,K^＋,Na^＋,P^5＋）,and changes in histopathology were observed after 24 days.Transforming growth factor-β1（TGF-β1） expression was examined by immuohistochemistry.Results： in ZG-Ⅲ treated group,the renal function is obviously lower than that in model group（P0.05）.ZG-Ⅲ increased the level of Ca2＋,reduced the levels of P5^＋and K^＋（P0.01）,TGF-β1expression level is lower compared with model group.Conclusion：Traditional Mongolian Midicine ZG-Ⅲ can protect renal function of adenine-induced CRF rats.the mechanism may be relative to suppressing the expression of TGF-β1.

关 键 词：章古-3汤 腺嘌呤 慢性肾功能衰竭(CRF) 转化生长因子β1(TGF-β1) 

分 类 号：P291.2[天文地球—测绘科学与技术] R961[医药卫生—药理学]