漆酶处理对杨木APMP木素分子质量的影响  

The Change of Lignin Molecular Weight of Aspen Alkaline Peroxide Mechanical Pulp Treated by Laccase and Laccase/Mediator

作　　者：崔红艳[1] 刘玉[1,2,3] 杨桂花[1] 王娟[1] Lucian A.Lucia 

机构地区：[1]山东轻工业学院制浆造纸工程省级重点学科,山东济南250353 [2]山东大学微生物技术国家重点实验室,山东济南250100 [3]美国北卡罗来纳州立大学木材与造纸科学系,美国罗利nc276958005

出　　处：《中国造纸学报》2011年第3期5-8,共4页Transactions of China Pulp and Paper

基　　金：国家自然科学基金项目(3097232631070525);山东自然科学基金项目(ZR2010CM065)的资助

摘　　要：研究了漆酶和漆酶/介体体系处理对三倍体毛白杨APMP性能的影响。采取酶解-弱酸解两段法分别从APMP原浆和生物处理后的纸浆中分离木素。为了使木素在凝胶渗透色谱流动相四氢呋喃中能完全溶解,对所有试样进行了卞基化处理。利用凝胶渗透色谱分析仪获得了木素的分子质量分布曲线,并计算出数均分子质量和质均分子质量,进而比较了漆酶和漆酶/介体体系处理的效果和差异。The alkaline peroxide mechanical pulps（APMP） of Triploid of Populus Tomentosa was treated by laccase and laccase-mediator-system（LMS） respectively,the factors affecting physical properties of the pulp were studied.The two-stage method of enzyme-mild acidic hydrolysis was adopted to isolate lignin from the APMP and the modified pulp samples.All the benzoylated lignin samples were dissolved in THF.The molecular weight distributions of lignins were obtained through GPC,the relative number average molecular weight and relative weight average molecular weight were figured out,and the differences of the effects of laccase and LMS treatments were compared.

关 键 词：木素 分子质量分布 漆酶 漆酶/介体体系 APMP 

分 类 号：TS711[轻工技术与工程—制浆造纸工程] Q55[生物学—生物化学]