灵杞黄斑颗粒对光损伤大鼠视网膜c-fos、caspase-3及bcl-xL表达的影响  

Effect of Lingqi Huangban Granule on c-fos,caspase-3 and bcl-xL Expression in Light-induced Retinal Damage of Rat

作　　者：宋毅[1] 宫媛媛[2] 吴星伟[2] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海交通大学附属第一人民医院,上海200080

出　　处：《四川中医》2011年第11期14-16,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：上海市科学技术委员会资助项目(NO:05dz22322)

摘　　要：目的:研究灵杞黄斑颗粒对大鼠光损伤模型视网膜细胞凋亡相关基因c-fos、caspase-3及bcl-xL表达的影响。方法:SD大鼠随机分为正常对照组、光损伤给水组、光损伤给药组。采用白色荧光灯持续照射5h建立光损伤模型,于光照2·5h、5h及光照后1d及3d,通过逆转录-聚合酶链反应(RT-PCR)和免疫蛋白印迹(western blot)分析视网膜c-fos、caspase-3及bcl-xL的转录表达。结果:c-fos、caspase-3mRNA和蛋白表达下调,bcl-xL mRNA和蛋白表达上调。结论:灵杞黄斑颗粒可调控光损伤大鼠视网膜细胞凋亡相关基因的表达。Objective： To investigate the effect of Lingqi Huangban Granule （LQHB） on apoptosis-associated c-los, caspase-3and bcl-xL expression in light-induced retinal damage of rat. Methods： Sprague-Dawley （SD） rats were randomly divided into three groups： normal control group, light damage with vehicle group and light damage with LQHB group, whose retinal light impairment was induced by exposure to white fluorescent constant light for 5 hours. At 2-5h, 5h, after ld and 3d light exposure, the mRNA and protein expression of c-fos, caspase-3, bcl-xL were observed by RT-PCR and western bolt methods. Results ： The mRNA and protein expression of c-fos and caspase-3was down regulated. The mRNA and protein expression of bcl-xL was up regulated. Conclusions ： LQHB could regulate the expression of apoptosis-associated gene in light-induced retinal damage of rat.

关 键 词：灵杞黄斑颗粒 光损伤 C-FOS CASPASE-3 BCL-XL 

分 类 号：R285.5[医药卫生—中药学]