野西瓜正丁醇提取物中化学成分研究  

Study of chemical constituents in n-butyl alcohol extract of Capparis spinosa L.

作　　者：谢乐琼[1,2] 于蕾[1,2,3] 于娜[1,2] 季宇彬[1,2,3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨150076 [3]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第5期659-661,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(30902013);黑龙江省自然科学基金项目(C200610)

摘　　要：对野西瓜Capparis spinosa L.中正丁醇提取物化学成分进行研究,为进一步探索野西瓜活性成分的分离提取方法及其充分开发利用提供科学依据.渗漉法阳离子交换树脂、索式提取法和硅胶柱色谱对野西瓜果实进行初步分离提取,结合反复硅胶柱色谱、Sephadex LH-20和重结晶技术对正丁醇部位的浸膏进行分离纯化,并采用光谱数据,色质联用等方法鉴定化合物.共得到3个化合物,即化合物(Ⅰ)尿嘧啶(uracil),化合物(Ⅱ)对羟基苯甲酸(p-hydroxybenzoic),化合物(Ⅴ)水苏碱(stachydrine)得到三个化合物为已知化合物,其中水苏碱为生物碱类活性成分.The chemical constituments in n-butyl alcohol extract of Capparis spinosa L.were studied.To provide scientific basis for further explore extract methods of chemical constituents of Capparis spinosa L.The percolation,soxhlet extractor and silica gel column chromatography were used to initially extract the fruit of Capparis spinosa L.The extract of n-butanol alcohol was separated and purificated by column chromatography with silica gel and Sephadex LH-20,and recrystallization.The compounds were identified on the basis of physicochemical constants,spectrum analysis and HPLC-MS.Three obtained compounds were compound Ⅰ（uracil）, Ⅱ（p-hydroxybenzoic acid）, Ⅲ（stachydrine）,respectively.The three compounds were known compounds.Compound Ⅲ（stachydrine） was an active ingredient of alkaloids.

关 键 词：野西瓜 生物碱 化学成分 结构鉴定 

分 类 号：R284[医药卫生—中药学]