Chaetoomium globosum与Panus rudis共培养发酵合成漆酶  

Laccase study on co-culture of Chaetoomium globosum and Panus rudis

作　　者：李静[1] 方泽民[2,3] 洪宇植[2,3] 金葆康[1] 肖亚中[2,3] 

机构地区：[1]安徽大学化学化工学院,安徽合肥230039 [2]安徽大学生命科学学院,安徽合肥230039 [3]安徽省微生物与生物催化工程技术研究中心,安徽合肥230039

出　　处：《安徽大学学报（自然科学版）》2011年第6期93-97,共5页Journal of Anhui University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31000032;30770058);安徽省自然科学基金资助项目(11040606Q04);安徽省教育厅自然科学基金重点资助项目(KJ2009A72)

摘　　要：研究Chaetoomium globosum与Panus rudis在共培养条件下的漆酶合成.平板对峙培养时,菌丝体生长呈僵持状态,接触区漆酶表达量提高;摇瓶发酵实验表明,C.globosum与P.rudis共培养时,漆酶活力达4 360 U.L-1(ABTS法),是P.rudis单独培养的14倍,而C.globosum单独培养时无漆酶分泌;改变初始发酵时添加的C.globosum菌丝体量,或者延长发酵时间,漆酶同工酶谱发生改变.Laccase production was analyzed during the co-culture of C.globosum and P.rudis.When P.rudis and C.globosum were paired on agar media,the mycelial fronts of the two species were deadlocked,and laccase activity increased during the interaction.Co-culture fermentation of two strains resulted in the increase of laccase activity,and reached 4 360 U·L-1,14 times higher than that of the control.However,no laccase activity was detected in liquid state culture stock of C.globosum.Extension of co-culture time or change the proportion of two fungi at the beginning of co-culture would change laccase isozyme of P.rudis.

关 键 词：漆酶 共培养 同工酶 

分 类 号：Q93[生物学—微生物学]