基于ITS、atpB-rbcL序列的醉翁榆(Ulmus gaussenii)与相近榆属植物的亲缘关系  

The phylogenetic relationships between Ulmus gaussenii Cheng and its close relatives in genus Ulmus based on the ITS and atpB-rbcL

作　　者：韩雪[1] 智颖飙[1] 张保卫[1] 周忠泽[1] 何家庆[1] 王再岚[2] 李红丽[3] 李静敏[1] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230039 [2]中国人民大学环境学院,北京100872 [3]北京林业大学自然保护区学院,北京100083

出　　处：《安徽大学学报（自然科学版）》2011年第6期98-107,共10页Journal of Anhui University(Natural Science Edition)

基　　金：中国博士后基金资助项目(20060400551);农业部草地开放实验室基金资助项目;安徽大学人才引进基金资助项目(06124;204033)

摘　　要：濒危植物保护生物学研究已成为生态学领域的研究热点之一.为了廓清我国特有濒危植物醉翁榆(Ulmus gaussenii)与相近榆属植物的系统地位,用分子标记ITS和atpB-rbcL手段分析醉翁榆与其他榆属相关种的亲缘关系,结果表明:24种榆属植物atpB-rbcL扩增片段长度为735～834 bp,G+C的含量在29.3%～31.6%之间,并没有发生很大的偏差;基于ITS(包括5.8 s)扩增片段,醉翁榆与黑榆系的毛果旱榆、栓枝春榆以及榆系中的大果榆、脱皮榆(U.lamellosa)相近;基于atpB-rbcL扩增片段,醉翁榆与榆系中的大果榆、脱皮榆以及明陵榆的关系比较近,两种标记都表现出醉翁榆与黑榆系中变种毛果旱榆、栓枝春榆的关系比较近;atpB-rbcL的NJ和Bayesian树完全支持榆属组下的分类以及榆属系统的演化关系.Conservation biology research of endanged plant has become popular in ecology area.To explore the systematic position of Ulmus gaussenii Cheng in genus Ulmus,the authors analyzed the phylogenetic relationship of U.gaussenii and its related species in Ulmus based on DNA sequences of the chloroplase atpB-rbcL gene and the ITS region of nuclear ribosomal DNA.The sequence of atpB-rbcL in 24 species ranged from 735 to 834 and the total G＋C content varied from 29.3% to 31.6%.The phylogenetic tree based on the atpB-rbcL data showed that U.gaussenii has near relationship with U.glaucescens var.lasiocarpa,U.japonica var.suberosa,U.macrocarpa,U.erythrocarpa.The phylogenetic tree based on the ITS data showed that U.gaussenii has near relationship with U.glaucescens var.lasiocarpa,U.japonica var.suberosa,U.macrocarpa,U.lamellose.During the analyzing process,the authors applied distance method and bayesian method,as they give more resolution and higher support value.Also,the species of interrelationships in genus are also discussed.

关 键 词：醉翁榆 遗传随机性 ATPB-RBCL ITS 系统树 

分 类 号：Q145[生物学—生态学] Q948[生物学—普通生物学]