亳菊叶片组织培养技术优化的研究  

Research on Tissue Culture Technology of Chrysanthemum morifolium Originated from Bozhou City

作　　者：陶兴魁[1,2] 张爱民[1] 薛建平[1] 薛涛[1] 卢河东[1] 

机构地区：[1]淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽淮北235000 [2]宿州学院化学与生命科学学院,安徽宿州234000

出　　处：《安徽农业科学》2011年第32期19671-19672,19691,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽高校省级自然科学研究重点项目(KJ2009A160);宿州学院特色种植业苗种生产工程技术研究中心项目(2011YKF18)

摘　　要：[目的]研究不同植物生长物质对诱导亳菊叶片愈伤组织诱导和不定芽分化的影响。[方法]以亳菊组培苗的叶片作为外植体,使用正接和背接2种方式接种于正交设计的培养基中,诱导叶片形成愈伤组织;再将愈伤组织接种于新配制的培养基中,对愈伤组织诱导率和芽分化率进行方差和极差分析。[结果]诱导亳菊叶片形成愈伤组织最佳的培养基组合为MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA+0.2 mg/L NAA;诱导愈伤组织分化成不定芽的最佳培养基是MS+2.0 mg/L 6-BA+0.5 mg/LNAA。[结论]为亳菊的遗传转化体系构建和诱变育种提供了高效的操作系统。[Objective]The aim was to study the effects of different plant growth substances on callus inducing and bud differentiation of Chrysanthemum morifolium leaf.[Method]With C.morifolium leaf as explant,it was inoculated into orthogonal-Designed culture medium with two ways and was induced to form callus;then the callus was inoculated into new culture medium,the variance and range analysis were carried out on callus inducing rate and bud differentiation rate.[Result]The optimum culture medium for inducing C.morifolium leaf to form callus is MS＋ 2.0 mg/L 2,4-D ＋ 2.0 mg/L 6-BA＋0.2 mg/L NAA;and the optimum culture medium for bud differentiation is MS ＋ 2.0 mg/L 6-BA＋ 0.5 mg/L NAA.[Conclusion]The study will provide high effective operation system for constructing genetic transformation system and breeding of C.morifolium.

关 键 词：亳菊 叶片 组织培养 

分 类 号：S132[农业科学—农业基础科学]