不同产地鲜姜乙醇浸提物化学成分分析及6-姜酚含量的测定  

Component Analysis on Ethanol Extract of RHIZOMA ZINGIBERIS RECENS from Different Producing Areas and Assay of 6-Gingerol

作　　者：吴文利[1] 郝小燕[1] 王兵 王道平[3] 王颖[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州味美食品工业有限公司,贵州贵阳550001 [3]贵州省中国科学院天然产物化学重点试验室,贵州贵阳550002

出　　处：《安徽农业科学》2011年第32期19786-19788,19824,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅工业攻关计划项目(黔科合GY字20103043)

摘　　要：[目的]分析比较不同产地鲜姜乙醇浸提物的化学成分并定量测定鲜姜中6-姜酚的含量。[方法]采用GC-MS分析不同产地鲜姜乙醇浸提物中的化学成分,采用HPLC法测定鲜姜中6-姜酚的含量。[结果]5个不同产地的鲜姜乙醇浸提物中分别鉴定出39、44、45、49、44种化合物,其主要成分为姜酚、姜烯酚类、姜酮酚类及姜油酮类等;由于产地不同,各产地鲜姜的乙醇浸提物组成成分及相对含量存在较大差异。姜酚类化合物在鲜姜中含量相对较高,约占总挥发油的30%左右。采用HPLC法测定鲜姜中6-姜酚的含量,结果显示,6-姜酚在0.024 8～0.124 mg/ml浓度范围内与峰面积呈良好线性关系(r=0.996 6,n=5),平均加样回收率为99.20%,RSD为1.26%。[结论]GC-MS法能快速、简便、高效的检测鲜姜乙醇浸提物的化学成分,HPLC测定法简便可靠、重复性好,可用于鲜姜中6-姜酚的含量测定。[Objective] To analyze the chemical components in ethanol extracts of RHIZOMA ZINGIBERIS RECENS from different producing areas and to assay the content of 6-gingerol.[Method] GC-MS analysis and HPLC method were respectively adopted to analyze the chemical components in ethanol extracts and assay the content of 6-gingerol.[Result] 39,44,45,49 and 44 compounds were respectively identified from RHIZOMA ZINGIBERIS RECENS that was collected from 5 producing areas,mainly including gingerol（accounted for 30% of total essential oils）,shogaols,paradols and zingerone.Because of different producing areas,there were significant differences regarding chemical components and their relative contents in tested samples.6-Gingerol presented a good linearity with peak area between the concentration of 0.024 8 and 0.124 mg/ml（r=0.996 6,n=5）.The average recovery was 99.20% with the RSD of 1.26%.[Conclusions] GC-MS was quick,simple,convenient and effective to analyze the composition of ethanol extracts.HPLC method was simple,reliable and reproducible to determine the content of 6-gingerol.

关 键 词：GC-MS HPLC法 鲜姜(RHIZOMA ZINGIBERIS RECENS) 乙醇浸提物 6-姜酚 含量测定 

分 类 号：TS411[农业科学—烟草工业]