通心络对低氧预适应小鼠神经元超微结构及内皮细胞自修复能力的影响  

Effects of Tongxinluo on neuron ultrastructure and endothelial cell self-repairing ability in hypoxia preconditioning mice

作　　者：吴相春[1,2] 来静[3] 吴相锋[2] 贾振华 魏聪 王宏涛 

机构地区：[1]河北以岭医药研究院,石家庄050035 [2]河北以岭医院,石家庄050091 [3]石家庄市第三医院,石家庄050017

出　　处：《中国应用生理学杂志》2011年第4期396-399,共4页Chinese Journal of Applied Physiology

基　　金：国家重点基础研究发展计划(973计划)资助项目(2005CB523301);国家十一五"重大新药创制项目":络病理论指导药物开发集成创新技术平台建设(009ZX09313-003)

摘　　要：目的:观察低氧预适应对小鼠脑皮层神经元和内皮细胞超微结构的改变及通心络的作用。方法:40只小鼠随机分为正常对照组、低氧组、低氧预适应组和通心络组(低氧预适应+通心络),利用重复低氧5次复制低氧预适应小鼠模型后,记录小鼠每次低氧耐受的时间,观察脑皮层神经元和血管内皮细胞超微结构的改变。结果:随着低氧次数的增加,预适应组和通心络组小鼠耐受时间增加(P<0.05或P<0.01)。与预适应组比较,通心络组小鼠耐受时间增加(P<0.05或P<0.01);低氧组小鼠脑皮层内皮细胞和神经元超微结构发生明显改变,预适应组小鼠脑组织神经元及血管内皮细胞超微结构改善,通心络组脑组织神经元及血管内皮细胞超微结构明显改善。结论:低氧预适应显示出机体具有强大的自修复能力,通心络能明显增强机体的自修复能力,提高自适应。Objective： To observe the uhrastmcture changes of cerebral cortex neuron and endothelial cell in hypoxia preconditioning mice and the effects of Tongxinluo （TXL, Chinese traditional medilihe）on them. Methods： Mice were randomly divided into 4 groups： control group, hypoxia group, hypoxia preconditioning （I-IP） group and Tongxinluo （TXL）group. The hypoxia preconditioning mice were exposed by repetitive hypoxia for 5 runs. The animal＇s tolerance time of each hypoxia run was recorded. The ultrastructure change of cerebral neuron and endothelial cell were studied by electron micmseope. Results： The hypoxic tolerance time in HP and TXL groups were significantly increased run by run. Compared with HP group, the tolerance time of TXL group were increased in every ran. The ultrastructure of cerebral neuron and endothelial cell in hypoxia group changed obviously, mitochondrion and endoplasmic reticulum destroyed. However they were slighter in HP group than those in hypoxia group. The change in TXL group had no obvious differentce with control group and were slighter than those in HP group. Conclusion： Hypoxia preconditioning shows that organism has a strong self-repairing ability. Tong xinluo self-repairing; could increase self-repairing ability and adaptive ability of mice to hypoxia obviously.

关 键 词：低氧预适应 通心络 自修复 神经元 内皮细胞 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学]