氧化应激在高原重体力劳动过程中急性高原反应发生中的作用  

Effect of oxidative stress in development of acute high altitude response during the process of strong physical work at high altitude

作　　者：杨生岳[1] 冯恩志[1] 闫自强[1] 贺巍[1] 田忠新[1] 殷和[1] 马力夫[1] 周其全[2] 石自福 

机构地区：[1]解放军第四医院兰州军区呼吸内科中心 [2]第三军医大学高原军事医学系 [3]解放军68303部队医院

出　　处：《中国应用生理学杂志》2011年第4期457-460,共4页Chinese Journal of Applied Physiology

基　　金：国家科技支撑计划项目(2009BA185B003)

摘　　要：目的:观察氧化应激在高原重体力劳动过程中急性高原反应(AHAR)发生中的作用。方法:由低海拔(1500 m)快速进入高原(3 700 m)并从事重体力劳动的男性官兵96名,年龄18～35岁。根据AHAR症状评分,分为重度AHAR组(A组,n=24)、轻中度AHAR组(B组,n=47)和无AHAR组(C组,n=25),在该高度逗留50 d后下撤前及返回低海拔(1 500 m)后12 h、15 d分别测定血清8-异前列腺素F2α(8-iso-PGF2α)、超氧化物歧化酶(SOD)、丙二醛(MDA),并与低海拔(1 500 m)50名健康官兵(D组)比较。结果:A组血清8-iso-PGF2α、MDA[分别为(9.53±0.47)μg/L、(8.91±0.39)μmol/L]水平显著高于B组[分别为(8.34±0.42)μg/L、(7.31±0.32)μmol/L]、C组[分别为(7.02±0.48)μg/L、(6.41±0.23)μmol/L和D组[分别为(5.13±0.56)μg/L、(5.48±0.33)μmol/L](均P<0.01),SOD(52.08±3.44)μ/mL水平显著低于B组(62.27±2.54)μ/mL、C组(71.99±3.35)μ/mL和D组(80.78±3.44)μ/mL,(均P<0.01),B组与C组之间和C组与D组之间亦有显著性差异(均P<0.01)。海拔3 700 m AHAR总计分与血清8-iso-PGF2α、MDA呈显著正相关(均P<0.01),与血清SOD显著负相关(P<0.01);8-iso-PGF2α、MDA与SOD显著负相关(均P<0.01)。海拔3 700 m 50 d,血清8-iso-PGF2α、MDA水平显著高于,SOD水平显著低于海拔1 500 m 12h、15 d和D组(均P<0.01),海拔1 500 m 12 h与15 d之间有显著性差异(均P<0.01),海拔1 500 m 15 d与D组之间无显著性差异。结论:人体在高原低氧并重体力时氧化应激和氧化-抗氧化失衡与AHAR的发病和程度有密切关系,氧化应激和氧化-抗氧化失衡越严重,AHAR越重。返回低海拔后12 h有显著改善,15 d恢复到正常水平。Objective： To assess the effect of oxidative stress in development of acute high altitude response （AHAR） during the process of strong physical work at high altitude and its change after return to lower altitude. Methods： Ninety-six officers and soldiers of rapid entering into high altitude （3 700 m） with strong physical work were analyzed, all subjects were male, aged 18 - 35 years. According to the symptomatic scores of AHAR were divided into 3 groups： severe AHAR （group A, n = 24）, mild AHAR （group B, n = 47） and without AHAR （group C, n = 25）. Levels in serum 8-iso prostaglandinF2α（8-iso-PGF2α）, superoxide dismutase（SOD） and malonaldehyde（MDA） were measured at higher altitude stayed 50 d and after return to lower altitude （1 500 m） 12 h and 15 d,and 50 healthy volunteers （group D） at 1 500 m altitude served as controll. Results： Levels of serum 8-iso-PGF2α and MDA[ （ 9.53 ± 0.47） μg/L, （8.91 ± 0.39 ） μmol/L] were significantly higher in group A than those in group B [ （8.34 ± 0.42）μg/L, （7.31 ± 0.32）μmol/L] , group C [ （7.02 ± 0.48）μg/L, （6.41 ±0. 23）μmol/L] and group D [ （5.13 ± 0.56）μg/L, （5.48 ± 0.33）μmol/L], （all P 〈 0.01）, and serum SOD [ （52.08 ± 3.44）μmol/L was significantly lower in group A than that in group B [62.27 ± 2.54）μ/ml], group C [ （71.99 ± 3.35）μ/ml] and group D [ （80.78 ± 3.44）μ/ ml] （all P 〈 0.01）, there were signitlcantl differences between group B and C, C and D （all P 〈 0.01） . At altitude 3 700 m 50 d,AHAR scores was positively correlated with serum 8-iso-PGF2a and MDA （all P 〈 0.01）, negatively correlated with SOD（ P 〈 0.01）. Serum 8-iso- PGF2a and MDA were negatively correlated with SOD （all P 〈 0.01）. Levels of serum 8-ise-PGF2α and MDA were significantly higher at altitude of 3 700 m 50 d than those at altitude of 1 500 m 12 h, 15 d in group D （all P 〈 0.01）, and serum SOD was significantly lower than that at 1 500 m 12

关 键 词：氧化应激 8-异前列腺素F2Α 超氧化物歧化酶 丙二醛 高原 

分 类 号：R594.3[医药卫生—内科学]