结核杆菌H37Ra感染巨噬细胞的研究  

Study on macrophages infected with Mycobacterium tuberculosis H37Ra in vitro

作　　者：刘来成[1] 王淑玲[2] 卢贤瑜[3] 曾佑群[1] 吴扬[1] 张鹏辉[1] 

机构地区：[1]重庆医科大学附属儿童医院临检中心,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆400014 [2]重庆市第三人民医院,重庆市临床检验中心,重庆400014 [3]重庆医科大学临床免疫学教研室,重庆400016

出　　处：《细胞与分子免疫学杂志》2011年第11期1195-1197,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:研究结核杆菌H37Ra感染巨噬细胞后氮氧化物的产生及细胞因子的分泌和表达情况。方法:结核杆菌H37Ra感染RAW264.7巨噬细胞株24 h后收集上清液,用Griess法检测一氧化氮(NO),化学法检测过氧化氢(H2O2)的产生,逆转录-聚合酶链反应(RT-PCR)法检测感染后巨噬细胞IL-12和TNF-αmRNA的表达,ELISA法检测细胞因子IL-12和TNF-α的含量。结果:结核杆菌H37Ra感染巨噬细胞后产生的NO和H2O2水平均显著升高,同时IL-12和TNF-α的分泌和表达也明显增加(P<0.05)。结论:结核杆菌H37Ra感染巨噬细胞后能产生大量的氮氧化物和抗结核细胞因子,从而产生有利于宿主的抗结核免疫应答。AIM： To study the production of nitric oxide and secretion of cytokines after infection of macrophages with Mycobacterium tuberculosis H37 Ra. METHODS： 24 hours after infection of RAW264.7 cells with Mycobacterium tuberculosis H3？Ra, the production of NO and H202 as well as the secretion levels of IL-12 and TNF-α in the supernatants of culture were determined by Griess method, chemical method and ELISA assay respectively. The expression of IL-12 and TNF-α mRNA in macrophages was detected by reverse transcription polymerase chain reaction （RT- PCR）. RESULTS： Macrophages infected with Mycobacterium tuberculosis H37Ra produced effectively more NO, H2O2, and enhanced the release of IL-12, TNF-α and the expression of IL-12, TNF-α mRNA （ P 〈 0.05）. CONCLUSION： Mycobacterium tuberculosis H37Ra can induce the production of more nitric oxide and cytokines which play important roles in the host immune response.

关 键 词：结核分枝杆菌 H37RA 巨噬细胞 氮氧化物 细胞因子 

分 类 号：R378.911[医药卫生—病原生物学]