辅助性T细胞17在人类免疫缺陷病毒合并结核感染致病中的作用  被引量:4

The role of T helper type 17 cells in the pathogenesis of HIV/tuberculosis-coinfected patients

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作  者:彭巧丽[1] 张明霞[1] 李桂英[1] 张洁云[1] 朱秀云[1] 刘映霞[1] 刘水腾[1] 卢洪洲[2] 周伯平[1] 王辉[1] 

机构地区:[1]深圳市第三人民医院肝病研究所,518020 [2]上海市公共卫生临床中心

出  处:《中华传染病杂志》2011年第10期600-604,共5页Chinese Journal of Infectious Diseases

基  金:国家科技部十一五重大专项(2008ZX10001-008);广东省自然科学基金资助项目(9151051501000032)

摘  要:目的观察HIV合并结核感染患者IL-17的表达情况,探讨Th17细胞在HIV合并结核感染致病中的作用。方法将54例确诊的HIV感染患者,分为单纯HIV感染组、HIV合并潜伏结核感染组和HIV合并结核感染组,采用全血胞内细胞因子染色方法,使用BD FACSCanto流式细胞仪检测各项指标,FACSDiva软件分析CD4^+IL-17^+T淋巴细胞及CD4^+IFNγ^+T淋巴细胞表达情况,组间比较采用独立样本配对t检验。结果3组患者CD4^+T淋巴细胞计数及病毒载量无明显区别;CD4^+IL-17^+T淋巴细胞表达百分比单纯HIV感染组为(1.40±1.01)%,HIV合并结核潜伏感染组为(1.29±0.86)%,两组比较差异无统计学意义(t=0.336,P〉0.05),但均显著高于合并结核感染组的(0.58±0.43)%(r=3.680,t=2.516,P〈0.05);3组患者之间CD4^+IFNγ^+T淋巴细胞表达百分比分别为(32.8±24.0)%、(40.3±21.9)%和(46.1±31.2)%,3组比较,差异无统计学意义(t=-0.939,t=-1.602,t=-0.646,均P〉0.05)。结论HIV合并结核感染患者出现明显的Th17应答下调,Th17细胞可能在HIV合并结核感染中起着重要的抗结核免疫保护作用。Objective To evaluate the ILl7 expression in HIV/tuberculosis coinfected patients and its role in the pathogenesis of this coinfeetion. Methods Fifty-four H1V infected patients were divided into three groups: simple HIV infected group, HIV with latent tuberculosis infection (HIV+ LTBI) group and HIV coinfected with active tuberculosis (HIV+ ATB) group. The whole blood intracellular cytokine staining was performed and samples were then detected by BD FACSCanto. The expressions of CD4 ^+ IL-17^+T cells and CD4^+ IFNγ^+ T cells were analyzed using FACSDiva software. Comparison between groups was done by independent sample t test. Results The CD4^+ T cell count and viral load among these three groups were comparable. There were no significant difference of the expression of CD4^+ IL-17^+ T cells between simple HIV infected group and HIV+LTBI group (1. 40± 1.01)% vs (1.29±0.86)tA, (t=0. 336, P〉0.05), but both of these two groups were much higher than HIV+ATBgroup (t=3.680,t=2.516,P〈0.05). There were no significant differences of the expression of CD4^+ IFNγ' T cells among these three groups [(32.8±24.0)% vs (40.3±21.9)% vs (,16.1±31. 2)%, (t=-0. 939,t=-1. 602,t=-0. 646, P〉0. 05)). Conclusion The Thl7 response is down-regulated in HIV/tuberculosis-coinfected patients, which may play an important antitubercular role in the pathogenesis of coinfection.

关 键 词:人类免疫缺陷病毒 T淋巴细胞 辅助诱导 结核 

分 类 号:R51[医药卫生—内科学]

 

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