辅助性T细胞17在人类免疫缺陷病毒合并结核感染致病中的作用  

The role of T helper type 17 cells in the pathogenesis of HIV/tuberculosis-coinfected patients

作　　者：彭巧丽[1] 张明霞[1] 李桂英[1] 张洁云[1] 朱秀云[1] 刘映霞[1] 刘水腾[1] 卢洪洲[2] 周伯平[1] 王辉[1] 

机构地区：[1]深圳市第三人民医院肝病研究所,518020 [2]上海市公共卫生临床中心

出　　处：《中华传染病杂志》2011年第10期600-604,共5页Chinese Journal of Infectious Diseases

基　　金：国家科技部十一五重大专项（2008ZX10001-008）;广东省自然科学基金资助项目（9151051501000032）

摘　　要：目的观察HIV合并结核感染患者IL-17的表达情况，探讨Th17细胞在HIV合并结核感染致病中的作用。方法将54例确诊的HIV感染患者，分为单纯HIV感染组、HIV合并潜伏结核感染组和HIV合并结核感染组，采用全血胞内细胞因子染色方法，使用BD FACSCanto流式细胞仪检测各项指标，FACSDiva软件分析CD4^＋IL-17^＋T淋巴细胞及CD4^＋IFNγ^＋T淋巴细胞表达情况，组间比较采用独立样本配对t检验。结果3组患者CD4^＋T淋巴细胞计数及病毒载量无明显区别；CD4^＋IL-17^＋T淋巴细胞表达百分比单纯HIV感染组为（1．40±1．01）％，HIV合并结核潜伏感染组为（1．29±0．86）％，两组比较差异无统计学意义（t=0．336，P〉0．05），但均显著高于合并结核感染组的（0．58±0．43）％（r=3．680，t=2．516，P〈0．05）；3组患者之间CD4^＋IFNγ^＋T淋巴细胞表达百分比分别为（32．8±24．0）％、（40．3±21．9）％和（46．1±31．2）％，3组比较，差异无统计学意义（t=-0．939，t=-1．602，t=-0．646，均P〉0．05）。结论HIV合并结核感染患者出现明显的Th17应答下调，Th17细胞可能在HIV合并结核感染中起着重要的抗结核免疫保护作用。Objective To evaluate the ILl7 expression in HIV/tuberculosis coinfected patients and its role in the pathogenesis of this coinfeetion. Methods Fifty-four H1V infected patients were divided into three groups： simple HIV infected group, HIV with latent tuberculosis infection （HIV＋ LTBI） group and HIV coinfected with active tuberculosis （HIV＋ ATB） group. The whole blood intracellular cytokine staining was performed and samples were then detected by BD FACSCanto. The expressions of CD4 ^＋ IL-17^＋T cells and CD4^＋ IFNγ^＋ T cells were analyzed using FACSDiva software. Comparison between groups was done by independent sample t test. Results The CD4^＋ T cell count and viral load among these three groups were comparable. There were no significant difference of the expression of CD4^＋ IL-17^＋ T cells between simple HIV infected group and HIV＋LTBI group （1. 40± 1.01）% vs （1.29±0.86）tA, （t=0. 336, P〉0.05）, but both of these two groups were much higher than HIV＋ATBgroup （t=3.680,t=2.516,P〈0.05）. There were no significant differences of the expression of CD4^＋ IFNγ＇ T cells among these three groups [（32.8±24.0）% vs （40.3±21.9）% vs （,16.1±31. 2）%, （t=-0. 939,t=-1. 602,t=-0. 646, P〉0. 05））. Conclusion The Thl7 response is down-regulated in HIV/tuberculosis-coinfected patients, which may play an important antitubercular role in the pathogenesis of coinfection.

关 键 词：人类免疫缺陷病毒 T淋巴细胞 辅助诱导 结核 

分 类 号：R51[医药卫生—内科学]