圆斑星鲽染色体与多种显带的形态特征分析  

Analysis of the karyotype and banding patterns of Verasper variegatus

作　　者：王妍妍[1] 柳学周[1] 刘新富[1] 徐永江[1] 孙中之[1] 兰功刚 

机构地区：[1]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071 [2]青岛忠海水产有限公司,山东青岛266414

出　　处：《水产学报》2011年第11期1607-1616,共10页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2006AA10A414;2010AA10A402);国家鲆鲽类产业技术体系建设专项资金(CAKS-50);公益性农业行业专项项目(nyhyzx07-046)

摘　　要：采用体内注射植物血细胞凝集素(PHA)和秋水仙素法制备圆斑星鲽染色体,研究其染色体C-带、Ag-NORs带及G-带的显带特征。结果发现,圆斑星鲽具有46条染色体,核型为2n=46t,染色体臂数为NF=46,所检个体全部为二倍体,未发现存在异型性染色体和随体染色体的现象。带型研究表明,C-带特征为46条染色体均有大小不一的C-带,其中第22对染色体整体呈阳性深染,第19对染色体具有端部C-带,其余均为着丝粒C-带;计算其异染色质含量为30%;Ag-NORs带型特征为具有1对Ag-NORs,位于2号染色体的长臂末端,为端部Ag-NORs;G-带特征为具有38条深染带,21条浅染带。研究结果为圆斑星鲽染色体组学和种质资源保护提供了基础资料。The karyotype of Verasper variegatus was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines.Metaphase chromosomes were analyzed by means of C-banding,Ag-NORs and G-banding.Experiment results showed that the diploid chromosome number of V.variegatus was 46（2n=46t,NF=46）.There was no visible evidence of sex chromosome and satellite chromosome.C-bands of V.variegatus were located mainly in the centromere regions of chromosomes except the No.19 and 22 chromosomes,and the 19th pair of chromosomes showed positive C-bands in the terminal region while the whole 22th pair of chromosomes bore positive C-band thoroughly.The C-banding heretochromatin accounts for 30% of the total chromosome surface in V.variegatus.Silver staining study showed that V.variegatus bore a pair of Ag-NORs which was located at the end of long arm of the 2nd chromosome.A total of 59 positive G-bands（38 dark,21 light）were recorded in 23 pairs of chromosomes.These results would provide basic data for the genomic research and biodiversity conservation of V.variegatus.

关 键 词：圆斑星鲽 染色体 C-带 AG-NORS带 G-带 

分 类 号：Q343.2[生物学—遗传学] S917[农业科学—水产科学]