三氯乙烯药疹样皮炎患者免疫相关基因的表达水平  

Study on mRNA expression of immune-re lated genes in patients with allergic dermatitis induced by trichloroethylene

作　　者：徐新云[1] 刘月峰[1] 易娟 柯跃斌[1] 袁建辉[1] 黄海燕[1] 毛吉炎[1] 毛侃琅[1] 

机构地区：[1]深圳市疾病预防控制中心现代毒理学重点实验室,518055 [2]深圳市职业病防治院检验科

出　　处：《中华劳动卫生职业病杂志》2011年第11期816-819,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：深圳市科技计划项目（200802073）

摘　　要：目的探讨三氯乙烯（TCE）药疹样皮炎患者外周血免疫相关基因Foxp3、GATA3、CTLA4、T—bet的mRNA表达。方法选取TCE药疹样皮炎患者8例为病例组，8例健康人为对照组，抽取两组对象的外周血，采用实时荧光定量PCR（real—timequantitativePCR）技术检测外周血免疫相关基因Foxp3、GATA3、CTLA4、T—bet的mRNA的表达水平。结果与对照组比较，病例组Foxp3、GATA3、CT—LA4基因mRNA表达水平分别升高115％、97％和241％，差异有统计学意义（P〈0．01）；与对照组比较，病例组T—bet基冈mRNA表达水平下降47％，差异有统计学意义（P〈O．01）。结论TCE药疹样皮炎的部分免疫相关基斟表达水平发生明显改变，这些基因可能在TCE引起的变态反应中发挥重要作用。Objective To study mRNA expression of immune-related genes （Foxp3, GATA3, CTLA4 and T-bet） in peripheral blood of the patients with allergic dermatitis induced by trichloroethylene （TCE）. Methods The peripheral blood samples were collected from 8 healthy workers （control group） and 8 patients with allergic dermatitis induced by TCE （case group）. Real-time quantitative PCR was applied to detect mRNA expression of immune-related genes （Foxp3, GATA3, CTLA4, T-bet）. Results The mRNA expression levels of Foxp3, GATA3 and CTLA4 genes increased by 115%, 97% and 241% in case group, as compared with con-trol group （P〈0.01）. The mRNA expression level of T-bet gene decreased by 47% in case group, as compared with control group （P〈0.01）. Conclusion The mRNA expression levels of some immune-related genes changed in patients with allergic dermatitis induced by TCE, those genes may play an important role in TCE-induced allergy.

关 键 词：三氯乙烯 皮炎 职业性 超敏反应 基因表达 

分 类 号：R758[医药卫生—皮肤病学与性病学]