和胃1号促进胃干细胞定向分化的动物实验研究  

Experimental Study of Hewei-Decotion in Promoting the Differentiation of Gastric Mucosa Stem Cell

作　　者：田萍[1] 党莹[1] 韩海明[1] 刘大锐[1] 高志星[2] 季万胜[2] 

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261053 [2]潍坊医学院附属医院消化内科

出　　处：《潍坊医学院学报》2011年第1期39-40,共2页Acta Academiae Medicinae Weifang

基　　金：山东省中医药科技发展计划项目（课题编号：2005237）

摘　　要：目的 探讨和胃1号促进胃干细胞定向分化的作用机制.方法 以水杨酸钠法复制慢性萎缩性胃炎（CAG）模型.免疫组化法检测各组胃黏膜腺体泌酸细胞数量;半定量实时荧光PCR法检测胃黏膜三叶因子（TFF）TFF1,TFF2和musashi-1 mRNA的表达;琼脂糖凝胶电泳检测表达产物的特异性.结果 模型组小鼠胃黏膜TFFI,TFF2 mRNA表达水平及泌酸细胞数量显著低于正常组（P〈0.05）,干预组TFF1,TFF2 mRNA的表达水平及泌酸细胞数量显著高于模型组（P〈0.05）.模型组小鼠胃黏膜musashi-1 mRNA表达水平显著高于干预组与正常组（P〈0.01）,干预组表达减少.结论 和胃1号可促进胃干细胞定向分化,主要作用机制为上调TFF1,TFF2表达,下调musashi-1表达.Objective To investigate the mechanism of Hewei-decotion in promoting the differentiation of gastric stem cell. Methods Chronie atrophic gastritis （CAG） model of mouse was made by Sodium Salicylate. The mice were randomly divided into three grnups,that was normnl control, CAG and Hewei-Deeoction intervention group. The expression of oxyntic cells was detected by immuno- histechemical method. Real-time semiquantitative polymerase chain reaction（PCR） was performed to detect the level of TFF1 ,TFF2 and mu- sashi-1 mRNA expression in gastric mueosa. Agarose gel eleetrophoresis was used to deteet the specialty of the amplified produets. Results The expression level of TFF1 , TFF2 mRNA and the number of oxyntic eells in CAG group were remarkably lower than that in the control group,which was improved significantly by the intervention of Hewei-decoetion （ P 〈 0.05 }. The expression level of musashi-1 mRNA was higher in CAG oup than in the other two groups. Conclusion Hewei-Deeoetion may enhance the differentiation of gastric mncosa stem cell, by upregulating TFFI ,TFF2 expression,and reducing expression of musashi-l.

关 键 词：胃干细胞 中医药疗法 和胃1号 三叶因子 MUSASHI-1 

分 类 号：R965[医药卫生—药理学]