鼠疫菌荚膜抗原和重组rV270抗原免疫小鼠后保护效果观察  

Observation of protective effect on mice after immunization with Yersinia pestis capsular antigen F1 and recombinant rV270

作　　者：戴瑞霞[1] 祁芝珍[1] 任玲玲[1] 张青雯[1] 李存香[1] 魏荣杰[1] 吴海莲[1] 王效义[2] 王祖郧[1] 

机构地区：[1]青海省地方病预防控制所鼠疫预防控制科,西宁811602 [2]军事医学科学院微生物流行病研究所

出　　处：《中国地方病学杂志》2011年第6期646-648,共3页Chinese Jouranl of Endemiology

基　　金：青海省应用基础研究及软科学计划项目（2008-Z-617）

摘　　要：目的对小鼠血清抗体滴度检测和对小鼠的保护力观察，评价鼠疫菌荚膜抗原（F1抗原）和重组rV270抗原对鼠疫的免疫保护效果。方法40只6—8周雌性Balb／e小鼠，按体质量随机分成4个实验组（F1-10μg＋铝佐剂、F1—20pg＋铝佐剂、rV-10μg＋铝佐剂、rV-20μg＋铝佐剂）和1个对照组，每组8只。将天然F1抗原和重组rV270抗原分别吸附到25％铝佐剂中免疫实验组小鼠，对照组小鼠以免疫等量的铝佐剂。每只动物每次后腿肌肉免疫100μl，初次免疫后，第21天加强免疫1次。所有动物分别于第1次免疫后第8周采血，酶联免疫吸附试验（ELISA）测定抗体滴度。同时用2000倍半数致死量（LD50）的鼠疫菌141强毒株皮下攻毒，观察14d，以观察免疫后保护效果。结果对照组未产生抗体，F1—10μg＋铝佐剂组、F1—20μg＋铝佐剂组的抗体几何平均滴度（GMT）分别为1：30443．9、1：21527．8，组间比较差异无统计学意义（t=1．1282，P〉0．05）；rV-10μg＋铝佐剂组和rV-20μg＋铝佐剂组的GMT分别为1：13957．3、1：18100．9，组间比较差异无统计学意义（t=0．9408，P〉0．05）。用141强毒株皮下攻毒后，实验组小鼠全部存活，对照组8只小鼠全部死亡。结论实验用天然F1抗原及重组rV270抗原具有较高的免疫活性，可作为鼠疫亚单位疫苗的主要组分用于鼠疫亚单位疫苗的研究。Objective To evaluate the protective effect of Yersinia pestis capsular antigen F1 and recombinant rV270 on mice after immunization with them. Methods According to body weight, 40 female Balb/c mice aged 6 to 8 weeks were randomly divided into four experimental groups（F1-10 μg ＋ aluminum adjuvant, F1- 20μg ＋ aluminum adjuvant, rV-10 μg ＋ aluminum adjuvant, and rV-20 μg ＋ aluminum adjuvant） and a control group, 8 in each group. Mice in experimental groups were immunized with the natural antigen F1 and recombinant antigen rV270 adsorbed to 25% aluminum adjuvant and the control group was immunized with the same amount of aluminum adjuvant. Each mouse was immunized at the hind leg muscle with 100 ml immunizing agent, then a booster immunization was done once on the 21st day after the first immunization. The blood of all mice was collected on the 8th week after the first immunization, serum antibody titers were detected by ELISA and the data of antibody titers were analyzed by t test for comparison between groups. At the same time the mice were injected subcutaneously with 2000-fold LD50 of Yersinia pestis virulent strain 141, after 14 days, the protective effect of immunization was analyzed. Results The control group did not produce antibody. Antibody geometric mean titers （GMT） of the FI-10 mg ＋ aluminum adjuvant and F1-20 mg ＋ aluminum adjuvant groups were 1 ： 30443.9, and 1 ： 21527.8, respectively, and compared between the two groups , the difference was not statistically significant（t = 1.1282, P 〉 0.05）. The GMTs of the rV-10 μg ＋ aluminum adjuvant and rV-20 μg ＋ aluminum adjuvant groups were 1 ： 13957.3 and 1 ： 18100.9, respectively, and compared between the two groups , the difference was not statistically significant（t = 0.9408, P 〉 0.05）. After subcutaneous injection with Yersinia pestis virulent strain 141, all mice died in the control group but all survived in the experimental group. Conclusion The immune activity of natural antigen FI and recombinant rV270

关 键 词：耶尔森菌 鼠疫 抗原 抗体 免疫 

分 类 号：R392[医药卫生—免疫学]