海带藻际微生物抗菌活性及其多样性的初步研究  

ANTIMICROBIAL ACTIVITY AND DIVERSITY OF MARINE BACTERIA IN PHYCOSPHERE OF LAMINARIA JAPONICA

作　　者：褚椒江[1,2] 朱鹏[1,2] 严小军[1,2] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,宁波315211 [2]宁波大学海洋生物工程重点实验室,宁波315211

出　　处：《水生生物学报》2011年第6期972-979,共8页Acta Hydrobiologica Sinica

基　　金：国家"836计划"(2007AA09Z412);国家自然科学基金(40906080);宁波大学科研基金(xkl09110);宁波大学人才工程项目(RCL2008016)资助

摘　　要：从浙江台州海域健康海带、病烂海带(Laminaria japonica)及其周围海水中分离纯化海带藻际微生物,利用双层琼脂扩散法对获得的菌株进行抗菌活性筛选,比较活性菌株与来源的相关性,并对具有抗菌活性的菌株进行16S rRNA系统发生学分析。结果显示,在分离的143株海带藻际微生物中,有42株细菌具有抗菌活性,其中来源于健康海带和海水的活性菌株比例(35%和29%)大于来源于病烂海带的细菌(23%)。对其中16株具有广谱抗菌活性的菌株进行的16S rRNA系统发生学分析显示,分别属于Pseudoalteromonas、Rahnella、Donghaeana、Bacillus和Exiguobacterium 5个属。为从藻际微环境的微生物多样性入手了解海带病烂机制以及寻找新的抗菌药物提供研究材料。Kelp(Laminaria japonica) is important economic farming seaweed in our country,rich in vitamins,protein,minerals,fiber,inorganic salts and other substances.But with the continuous expansion of seaweed farming,rotten dis-eases that occurred during the breeding often cause significant economic losses.Rotten diseases are complex problems,relative researches have been carried out for many years,but yet there has not been a unified conclusion.Previously mainly method was to search for a single pathogen and develop corresponding inhibitors.With the theory of phyco-sphere and phycosphere microbes,the balance of phycosphere micro-environment was found important to the normal growth of algae,making it possible to study rotten diseases from the way of microbial diversity.In this study,marine bacteria were isolated from health kelps(Laminaria japonica),rotten diseased kelps and seawater in coastal area of Taizhou,China.Antibacterial activities were screened using agar diffusion method,and the relationship of active bacte-ria and their origin was studied.The results showed that 42 strains among total 143 isolated marine bacteria had antim-icrobial activity,which indicated that marine bacteria in phycosphere were extremely rich in anti-bacterial resources.The proportion of active bacteria associated with health kelps and seawater(35% and 29%,respectively) was higher than that isolated from rotten diseased kelps(23%).Molecular phylogenetic analysis of marine bacteria with broad-spectrum antibacterial activities based on 16S rRNA sequences indicated that they belonged to the genera of Pseudoal-teromonas,Rahnella,Donghaeana,Bacillus and Exiguobacterium,respectively.These results proved that marine bac-teria in phycosphere had great diversity in class and quantity,while different inhibitory effect to seven sensitive indica-tor bacteria meant there was also diversity in function.Diversity condition changed as the growth state of kelp changed.This study provides a clue to understand the mechanism of rotten disease from microbial div

关 键 词：海带 藻际 抗菌活性 多样性 

分 类 号：Q938.8[生物学—微生物学]