20℃下急性缺氧胁迫对红耳龟糖类代谢和抗氧化防御系统的影响  

EFFECTS OF ACUTE HYPOXIA STRESS ON CARBOHYDRATE METABOLISM AND ANTIOXIDANT DEFENSE AT 20℃ IN THE TURTLE TRACHEMYS SCRIPTS ELEGANS

作　　者：叶双[1] 黄来旺[1] 崔琳[1] 王梦霞[1] 黄晶晶[1] 冯照军[1] 

机构地区：[1]徐州师范大学生命科学学院,徐州221116

出　　处：《水生生物学报》2011年第6期1005-1011,共7页Acta Hydrobiologica Sinica

基　　金：徐州师范大学自然科学基金(No.05PYL05)资助

摘　　要：淡水红耳龟Trachemys scripts elegans是研究耐缺氧的模式动物之一,其对缺氧胁迫具有较完善的应激机制。该文研究了20℃下急性缺氧胁迫对红耳龟的糖类代谢和抗氧化防御系统的影响,以探讨红耳龟对急性缺氧胁迫的适应。采用生物化学方法对不同组织中的乳酸、糖原、血糖、Na+-K+-ATP酶、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)进行测定。结果显示,随着缺氧时间的延长,肝脏和骨骼肌糖原逐渐被消耗,肝脏、心肌和骨骼肌乳酸含量逐渐升高,血乳酸和血糖含量急速上升,ATP酶活性有所下降;SOD和CAT活性先升高后回落,MDA含量也相应地变化。结果提示,龟在20℃急性缺氧环境中,糖类以无氧呼吸方式快速分解,抗氧化防御系统及时启动清除氧自由基。The survival strategy of animals in environment stress is one of ecology hot projects for scholars to study nowadays,the problem that biochemical physiology responses of animals to hypoxia environment is focused by scholars for long-term.As a famous model,the freshwater red-eared turtle,Trachemys scripts elegans,is one of the hy-poxia-tolerant animals for studying in biology and medicine field,especially at lower temperature,and it has more ex-cellent mechanisms to adapt hypoxia stress.Effects of acute hypoxia stress on carbohydrate metabolism and antioxidant defense of red-eared turtle was studied at 20 ℃,and the adaptation of the turtle to acute hypoxia stress was discussed in this paper.Within the thermostat at 20 ℃,the red-eared turtles were randomly divided into four test groups(at 3,6,12 and 24h respectively of hypoxia in the containers with oxygen-poor water) and one control group(at 0h).We took out five turtles respectively on time,the blood was collected from its heart,then the liver,cardiac muscle and skeletal mus-cle were cut,and tissue homogenate solution was prepared as normal method.The lactate consistency,glycogen content,plasma glucose level,Na+-K+-ATPase activity,superoxide dismutase(SOD) activity,catalase(CAT) activity and malonaldehyde(MDA) content of different tissues were assayed by using biochemical methods.After hypoxia-treatment,the phenomenon showed that the turtle’s struggle aggravated gradually in early hypoxia,but the struggle weakened gradually in later hypoxia.After 27h hypoxia-treatment,the turtles died successively.The turtle’s heart rate of each test group was markedly slower than that of control group,and the longer the hypoxia-time was,the slower of heart rate was.Results of biochemistry indexes indicated that as the hypoxia time prolonged,compared respectively with control group,liver glycogen(reduced by 23%,36%,57% and 74%) and muscle glycogen(reduced by 19%,31%,43% and 58%) were depleted gradually,liver lactate(increased by 1.3,2.4,2.8 and 3.1 fold),muscle lactate(inc

关 键 词：急性缺氧胁迫 无氧呼吸 红耳龟 糖类代谢 抗氧化防御系统 

分 类 号：Q959.63[生物学—动物学] Q494