抗原负载的DC诱导CIK对MDA-MB-231细胞的杀伤活性研究  

Investigation on the killing activity of cytokine-induced killer cells induced by DC and pulsed with antigen in MDA-MB231 cells

作　　者：王妍柏[1] 杨宝珍[2] 陈耀平[2] 潘月英[2] 杨芝红[2] 张成磊[1] 

机构地区：[1]宁夏医科大学检验学院,银川750004 [2]宁夏医科大学总院医学实验中心,银川750004

出　　处：《现代免疫学》2011年第6期486-489,共4页Current Immunology

基　　金：宁夏银川市科技攻关项目(2009年)

摘　　要：探讨乳腺癌相关抗原负载后树突状细胞(DC)联合细胞因子诱导的杀伤细胞(CIK)对MDA-MB-231乳腺癌细胞株的杀伤效应。采用密度梯度离心法获取外周血单个核细胞(PBMC),常规法诱导DC、CIK细胞;流式细胞技术分析测定细胞表型;用反复冻融法制备MDA-MB-231细胞的相关抗原,并测定抗原浓度;MTT法分别测定在四个不同效靶比时CIK、DC+CIK和抗原负载的DC+CIK三组效应细胞对MDA-MB-231乳腺癌细胞的杀伤活性。结果:CIK细胞分别与DC和Ag-DC共培养后,可见CD3+CD56+双阳性表达细胞较培养前显著增加(P<0.05);将负载肿瘤相关抗原的DC与CIK共培养后,杀瘤活性明显提高(P<0.05)。提示,抗原负载的DC与CIK细胞共同孵育培养后,在相同效靶比时,对MDA-MB-231乳腺癌细胞杀伤效应与CIK细胞和DC-CIK两组效应细胞相比有显著提高,表明抗原负载后可提高CIK细胞对肿瘤细胞的杀伤活性,为乳腺癌临床生物免疫治疗提供实验室基础。To investigate the killing effect of cytokine-induced killer（CIK） loaded with cell associated antigen on MAD-MB231 cell line,the peripheral blood mononuclear cells（PBMC） were obtained by density gradient centrifugation method,while the dendritic cells（DC） and CIK cells were induced by routine methods.MTT was used to detect the killing effect of three groups of cell proportions CIK,DC＋CIK,antigen loaded DC＋CIK MAD-MB231 under 4 different effector-target ratios.It was found that the number of CD3＋/CD56＋ double-expressed cella was significantly elevated when the CIK cells were co-cultivated with with DCs and DCs loaded with antigen respectively（P0.05）.Also,the tumor killing activity of CIK cells was significantly increased when they were co-cultivated with DCs pulsed with antigen.These results may provide experimental basis for the immunotherapy of breast cancer.

关 键 词：乳腺癌 MDA-MB-231细胞株 肿瘤抗原 DC CIK 

分 类 号：R392[医药卫生—免疫学]