CD3＋CD56＋NKT细胞与Vα24~＋iNKT细胞的检测及其表型特点分析  

CD3^+CD56^+NKT lymphocytes and CD3^+TCRVα24^+invariant NKT lymphocytes: two different NKT cell subsets

作　　者：姚春艳[1] 姜丽娜[2] 邱晓静[1] 李柏青[1] 

机构地区：[1]蚌埠医学院免疫学教研室 安徽省感染与免疫重点实验室 [2]蚌埠医学院病理生理学教研室,蚌埠233000

出　　处：《现代免疫学》2011年第6期490-494,共5页Current Immunology

基　　金：安徽省教育厅科研项目(KJ2011Z253);蚌埠医学院科研项目(BY0917)

摘　　要：为了比较CD3+CD56+NKT细胞与CD3+TCRVα24+iNKT细胞在外周血淋巴细胞中的相对比例及其表面分子表达的差别,本研究采集了健康人外周全血,用四色荧光抗体染色和流式细胞术检测CD3+CD56+NKT细胞和CD3+TCRVα24+iNKT细胞在淋巴细胞中的比例,及其亚群表型及活化分子CD69的表达情况。检测结果表明,在正常人外周血淋巴细胞中CD3+CD56+NKT细胞所占比例为3.90%±2.89%,以CD8亚群占多数(57.61%±17.35%);而CD3+TCRVα24+iNKT细胞所占比例仅为0.39%±0.19%,且以CD4亚群占多数(56.60%±19.66%)。两种NKT细胞的相对数量之间存在显著正相关(r=0.467,P<0.05),但两类细胞之间极少重叠。CD16和CD161在CD3+CD56+NKT细胞上的表达量显著高于在CD3+TCRVα24+iNKT细胞上的表达量(P均<0.01)。活化分子CD69在两种细胞上的表达量均较低(P>0.05)。本研究结果表明,正常人外周血CD3+CD56+NKT细胞与CD3+TCRVα24+iNKT细胞在相对数量、亚群及表型上存在显著差异,是两种截然不同的NKT细胞。In order to compare the frequency and surface phenotype of CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋ invariant NKT（iNKT） cells in human peripheral blood lymphocytes,the peripheral whole blood samples from 23 healthy donors were obtained and stained with four-color fluorescence conjugated antibodies.The flow cytometry was performed to detect the frequency,surface phenotype of subsets and activation on CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋ iNKT cells.The results showed that the proportion of CD3＋CD56＋NKT cells in the peripheral blood lymphocytes was 3.90±2.89%,of which the proportion of CD8 positive subset was 7.61%±17.35%.Compared to CD3＋CD56＋NKT cells,the proportion of CD3＋ TCRVα24＋ iNKT cells was 0.39±0.19%,the major subset of iNKT cells was CD4 positive subset 56.60±19.66%.There was a positive correlation between the proportion of CD3＋CD56＋NKT cells and CD3＋ TCRVα24＋ iNKT cells（r=0.467,P0.05）,but little or no overlap between the two cell subsets.The expressions of CD16 and CD161 on CD3＋CD56＋NKT cells were 12.24±10.09% and 55.94±20.96%,respectively oth significantly higher than that on CD3＋ TCRVα24＋iNKT cells（3.2±2.38% and 33.95±14.14%,）（P0.01）.The expression of CD69 was both low on these two cell subsets.These results demonstrate the CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋iNKT cells are two distinct NKT cell subsets owing to the significant differences of frequency and surface phenotype of subsets of the two cell subpopulations in human peripheral blood.

关 键 词：NKT细胞 INKT细胞 CD16 CD161 

分 类 号：R392[医药卫生—免疫学]